Brilliant blue in vitreoretinal surgery

Research output: Chapter in Book/Report/Conference proceedingChapter

29 Citations (Scopus)

Abstract

This paper reviews the preclinical effects of brilliant blue G (BBG) on the morphology and functions of the retina, and reports on a pilot study of BBG staining and subsequent peeling of the internal limiting membrane (ILM) during vitreoretinal surgery. BBG solution was injected into rat eyes and investigated using light microscopy and electron microscopy, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining, and electroretinography (ERG). No pathological changes were caused by the BBG intravitreous injection. Although electron microscopy revealed that high doses of BBG induced vacuolization in the inner retinal cells, there was no reduction in the amplitude of the ERG waves and no detectable toxic effects. In the primate eyes, the ILM was clearly visualized by BBG staining, and peeled off easily from the retina. In the clinical study, BBG improved visualization of the ILM, allowing peeling and surgery to be performed successfully on patients with various vitroretinal diseases. Improvement of postoperative visual acuity was obtained in most cases, and no adverse effects were observed postoperatively. In conclusion, BBG has low toxicity, high staining ability, and is easy to handle, making it a good candidate dye for visualizing vitreoretinal disease surgery without adverse effects.

Original languageEnglish
Title of host publicationVital Dyes in Vitreoretinal Surgery
Subtitle of host publicationChromovitrectomy
EditorsCarsten Meyer
Pages115-125
Number of pages11
DOIs
Publication statusPublished - Aug 20 2008

Publication series

NameDevelopments in Ophthalmology
Volume42
ISSN (Print)0250-3751
ISSN (Electronic)1662-2790

Fingerprint

Vitreoretinal Surgery
Staining and Labeling
Electroretinography
Membranes
Retina
Electron Microscopy
Aptitude
DNA Nucleotidylexotransferase
brilliant blue
coomassie Brilliant Blue
Poisons
Primates
Visual Acuity
Microscopy
Coloring Agents
Light
Injections

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Enaida, H., & Ishibashi, T. (2008). Brilliant blue in vitreoretinal surgery. In C. Meyer (Ed.), Vital Dyes in Vitreoretinal Surgery: Chromovitrectomy (pp. 115-125). (Developments in Ophthalmology; Vol. 42). https://doi.org/10.1159/000138989

Brilliant blue in vitreoretinal surgery. / Enaida, H.; Ishibashi, Tatsuro.

Vital Dyes in Vitreoretinal Surgery: Chromovitrectomy. ed. / Carsten Meyer. 2008. p. 115-125 (Developments in Ophthalmology; Vol. 42).

Research output: Chapter in Book/Report/Conference proceedingChapter

Enaida, H & Ishibashi, T 2008, Brilliant blue in vitreoretinal surgery. in C Meyer (ed.), Vital Dyes in Vitreoretinal Surgery: Chromovitrectomy. Developments in Ophthalmology, vol. 42, pp. 115-125. https://doi.org/10.1159/000138989
Enaida H, Ishibashi T. Brilliant blue in vitreoretinal surgery. In Meyer C, editor, Vital Dyes in Vitreoretinal Surgery: Chromovitrectomy. 2008. p. 115-125. (Developments in Ophthalmology). https://doi.org/10.1159/000138989
Enaida, H. ; Ishibashi, Tatsuro. / Brilliant blue in vitreoretinal surgery. Vital Dyes in Vitreoretinal Surgery: Chromovitrectomy. editor / Carsten Meyer. 2008. pp. 115-125 (Developments in Ophthalmology).
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