Broad reacting surface antigens in leptospira biflexa serovar andamana

B. Cacciapuoti, L. Ciceroni, T. Saito, E. Ono, R. Yanagawa

Research output: Contribution to journalArticle

Abstract

Previous investigations had demonstrated that genus-specific and species-specific antigens of leptospires are deep-seated within the leptospiral cell. Conversely, the present study has shown that strain CH11, serovar andamana of the non-pathogenic species of Leptospira biflexa is endowed on its surface with two cross-reacting antigens; a newly recognized antigen common to L. interrogans and L. biflexa spp. and an antigenic determinant common to a previously described genus-specific protein antigen (GP-Ag). The serovar andamana, when thimerosal-treated, behaved like an interspecies-specific antigen, cross-reacting in the complement fixation test with sera from rabbits immunized with L. interrogans and L. biflexa spp. and with sera from subjects with leptospirosis from various serovars. In the immuno electron microscopic test, human leptospirotic sera bound to the surface of thimerosal-treated andamana and not to the surface of untreated andamana showing that an interspecies-specific antigen was located underneath the outermost layer of this serovar. In the same test, the monoclonal antibody GP-7 against the GP-Ag bound to the surface of untreated andamana and not to the surface of thimerosal-treated andamana, showing that an antigenic determinant, common to GP-Ag and different from the first one, was located on the outer membrane of andamana. In human leptospirotic sera, antibodies against the cross-reacting antigen of thimerosal-treated andamana demonstrated by the complement fixation test were formed earlier and in a higher percentage of sera than the serovar-specific antibodies against 16 L.interrogans serovars demonstrated by the microagglutination test.

Original languageEnglish
Pages (from-to)86-99
Number of pages14
JournalZentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A
Volume269
Issue number1
DOIs
Publication statusPublished - Jan 1 1988
Externally publishedYes

Fingerprint

Leptospira
Surface Antigens
Antigens
Thimerosal
Complement Fixation Tests
Serum
Epitopes
Serogroup
Leptospirosis
Proteins
Antibodies
Monoclonal Antibodies
Electrons
Rabbits
Membranes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Broad reacting surface antigens in leptospira biflexa serovar andamana. / Cacciapuoti, B.; Ciceroni, L.; Saito, T.; Ono, E.; Yanagawa, R.

In: Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A, Vol. 269, No. 1, 01.01.1988, p. 86-99.

Research output: Contribution to journalArticle

Cacciapuoti, B. ; Ciceroni, L. ; Saito, T. ; Ono, E. ; Yanagawa, R. / Broad reacting surface antigens in leptospira biflexa serovar andamana. In: Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A. 1988 ; Vol. 269, No. 1. pp. 86-99.
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