BUBR1 insufficiency is correlated with eNOS reduction experimentally in vitro and in vivo, and in gastric cancer tissue

Eisuke Kawakubo, Takuya Matsumoto, Keiji Yoshiya, Sho Yamashita, Tomoko Jogo, Hiroshi Saeki, Eiji Oki, Tadashi Furuyama, Yoshinao Oda, Yoshihiko Maehara

Research output: Contribution to journalArticle

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Abstract

Background/Aim: Budding uninhibited by benzimidazole-related 1 (BUBR1) and endothelial nitric oxide synthase (eNOS) are related to aging and angiogenesis. This study examined the effect of low BUBR1 expression on eNOS expression in vivo, in vitro, and human gastric cancer tissues. Materials and Methods: Human umbilical vein endothelial cells (HUVECs) were passaged to investigate the effect of aging on BUBR1 and eNOS expression; expression of eNOS and phospho-eNOS protein was assessed in BUBR1 siRNAtransfected HUVECs. Additionally, guanosine 3′,5′ cyclic monophosphate (cGMP) and eNOS protein levels were measured in BUBR1-insufficient mice (Bubr1L/-). BUBR1 and eNOS expression levels were also evaluated in human gastric cancer tissues. Results: BUBR1 and eNOS, but not p-eNOS, levels were reduced significantly in aged and BUBR1 siRNAtransfected HUVECs. Additionally, cGMP production and the eNOS protein level were reduced in Bubr1L/- mice. Human gastric cancer tissues with low BUBR1 expression showed no eNOS expression. Conclusion: A decrease in BUBR1 reduced eNOS bioavailability through a pathway other than eNOS phosphorylation.

Original languageEnglish
Pages (from-to)6099-6106
Number of pages8
JournalAnticancer research
Volume38
Issue number11
DOIs
Publication statusPublished - Nov 1 2018

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Nitric Oxide Synthase Type III
Stomach Neoplasms
Human Umbilical Vein Endothelial Cells
In Vitro Techniques
benzimidazole
Proteins
Guanosine
Biological Availability

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kawakubo, E., Matsumoto, T., Yoshiya, K., Yamashita, S., Jogo, T., Saeki, H., ... Maehara, Y. (2018). BUBR1 insufficiency is correlated with eNOS reduction experimentally in vitro and in vivo, and in gastric cancer tissue. Anticancer research, 38(11), 6099-6106. https://doi.org/10.21873/anticanres.12960

BUBR1 insufficiency is correlated with eNOS reduction experimentally in vitro and in vivo, and in gastric cancer tissue. / Kawakubo, Eisuke; Matsumoto, Takuya; Yoshiya, Keiji; Yamashita, Sho; Jogo, Tomoko; Saeki, Hiroshi; Oki, Eiji; Furuyama, Tadashi; Oda, Yoshinao; Maehara, Yoshihiko.

In: Anticancer research, Vol. 38, No. 11, 01.11.2018, p. 6099-6106.

Research output: Contribution to journalArticle

Kawakubo, Eisuke ; Matsumoto, Takuya ; Yoshiya, Keiji ; Yamashita, Sho ; Jogo, Tomoko ; Saeki, Hiroshi ; Oki, Eiji ; Furuyama, Tadashi ; Oda, Yoshinao ; Maehara, Yoshihiko. / BUBR1 insufficiency is correlated with eNOS reduction experimentally in vitro and in vivo, and in gastric cancer tissue. In: Anticancer research. 2018 ; Vol. 38, No. 11. pp. 6099-6106.
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author = "Eisuke Kawakubo and Takuya Matsumoto and Keiji Yoshiya and Sho Yamashita and Tomoko Jogo and Hiroshi Saeki and Eiji Oki and Tadashi Furuyama and Yoshinao Oda and Yoshihiko Maehara",
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AU - Kawakubo, Eisuke

AU - Matsumoto, Takuya

AU - Yoshiya, Keiji

AU - Yamashita, Sho

AU - Jogo, Tomoko

AU - Saeki, Hiroshi

AU - Oki, Eiji

AU - Furuyama, Tadashi

AU - Oda, Yoshinao

AU - Maehara, Yoshihiko

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