Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space

Makiko Negishi, Takahiro Sakaue, Kingo Taniguchi, Kenichi Yoshikawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

It is known that actin play the essential role in the regulation of the structure and function in cellular and cell nuclei systems. DNA and actin filaments are the two major components of cell nuclei. We developed an artificial cell nuclei model with T4 DNA and F-actin in phospholipid-coated micro-meter scale closed spheres (MPDs). In a MPD, the mixture of T4 DNA and F-actin was found to phase-separate into F-actin phase (Px) and T4 DNA rich phase (P2), where the manner of the segregation is dependent on the total volume fraction of F-actin and T4 DNA. In addition, we found that, in a MPD, T4 DNA forms bundles in a crowded micro-space with F-actin even in the absence of multivalent cations.

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
Pages60-64
Number of pages5
DOIs
Publication statusPublished - Dec 1 2008
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan
Duration: Nov 6 2008Nov 9 2008

Publication series

Name2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

Other

Other2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CountryJapan
CityNagoya
Period11/6/0811/9/08

All Science Journal Classification (ASJC) codes

  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

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    Negishi, M., Sakaue, T., Taniguchi, K., & Yoshikawa, K. (2008). Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics" (pp. 60-64). [4752423] (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008). https://doi.org/10.1109/MHS.2008.4752423