Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space

Makiko Negishi, Takahiro Sakaue, Kingo Taniguchi, Kenichi Yoshikawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

It is known that actin play the essential role in the regulation of the structure and function in cellular and cell nuclei systems. DNA and actin filaments are the two major components of cell nuclei. We developed an artificial cell nuclei model with T4 DNA and F-actin in phospholipid-coated micro-meter scale closed spheres (MPDs). In a MPD, the mixture of T4 DNA and F-actin was found to phase-separate into F-actin phase (Px) and T4 DNA rich phase (P2), where the manner of the segregation is dependent on the total volume fraction of F-actin and T4 DNA. In addition, we found that, in a MPD, T4 DNA forms bundles in a crowded micro-space with F-actin even in the absence of multivalent cations.

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
Pages60-64
Number of pages5
DOIs
Publication statusPublished - Dec 1 2008
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan
Duration: Nov 6 2008Nov 9 2008

Publication series

Name2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

Other

Other2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CountryJapan
CityNagoya
Period11/6/0811/9/08

Fingerprint

DNA
Cells
Phospholipids
Volume fraction
Positive ions

All Science Journal Classification (ASJC) codes

  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

Cite this

Negishi, M., Sakaue, T., Taniguchi, K., & Yoshikawa, K. (2008). Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics" (pp. 60-64). [4752423] (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008). https://doi.org/10.1109/MHS.2008.4752423

Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space. / Negishi, Makiko; Sakaue, Takahiro; Taniguchi, Kingo; Yoshikawa, Kenichi.

2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. p. 60-64 4752423 (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Negishi, M, Sakaue, T, Taniguchi, K & Yoshikawa, K 2008, Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space. in 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"., 4752423, 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, pp. 60-64, 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation", Nagoya, Japan, 11/6/08. https://doi.org/10.1109/MHS.2008.4752423
Negishi M, Sakaue T, Taniguchi K, Yoshikawa K. Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. p. 60-64. 4752423. (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008). https://doi.org/10.1109/MHS.2008.4752423
Negishi, Makiko ; Sakaue, Takahiro ; Taniguchi, Kingo ; Yoshikawa, Kenichi. / Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space. 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. pp. 60-64 (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008).
@inproceedings{872d51e190784a1ca31728a22579eb27,
title = "Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space",
abstract = "It is known that actin play the essential role in the regulation of the structure and function in cellular and cell nuclei systems. DNA and actin filaments are the two major components of cell nuclei. We developed an artificial cell nuclei model with T4 DNA and F-actin in phospholipid-coated micro-meter scale closed spheres (MPDs). In a MPD, the mixture of T4 DNA and F-actin was found to phase-separate into F-actin phase (Px) and T4 DNA rich phase (P2), where the manner of the segregation is dependent on the total volume fraction of F-actin and T4 DNA. In addition, we found that, in a MPD, T4 DNA forms bundles in a crowded micro-space with F-actin even in the absence of multivalent cations.",
author = "Makiko Negishi and Takahiro Sakaue and Kingo Taniguchi and Kenichi Yoshikawa",
year = "2008",
month = "12",
day = "1",
doi = "10.1109/MHS.2008.4752423",
language = "English",
isbn = "9781424429196",
series = "2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008",
pages = "60--64",
booktitle = "2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on {"}COE for Education and Research of Micro-Nano Mechatronics{"}",

}

TY - GEN

T1 - Bundle formation in a crowded environment with giant DNA and F-actin inside a micro-meter scale closed-space

AU - Negishi, Makiko

AU - Sakaue, Takahiro

AU - Taniguchi, Kingo

AU - Yoshikawa, Kenichi

PY - 2008/12/1

Y1 - 2008/12/1

N2 - It is known that actin play the essential role in the regulation of the structure and function in cellular and cell nuclei systems. DNA and actin filaments are the two major components of cell nuclei. We developed an artificial cell nuclei model with T4 DNA and F-actin in phospholipid-coated micro-meter scale closed spheres (MPDs). In a MPD, the mixture of T4 DNA and F-actin was found to phase-separate into F-actin phase (Px) and T4 DNA rich phase (P2), where the manner of the segregation is dependent on the total volume fraction of F-actin and T4 DNA. In addition, we found that, in a MPD, T4 DNA forms bundles in a crowded micro-space with F-actin even in the absence of multivalent cations.

AB - It is known that actin play the essential role in the regulation of the structure and function in cellular and cell nuclei systems. DNA and actin filaments are the two major components of cell nuclei. We developed an artificial cell nuclei model with T4 DNA and F-actin in phospholipid-coated micro-meter scale closed spheres (MPDs). In a MPD, the mixture of T4 DNA and F-actin was found to phase-separate into F-actin phase (Px) and T4 DNA rich phase (P2), where the manner of the segregation is dependent on the total volume fraction of F-actin and T4 DNA. In addition, we found that, in a MPD, T4 DNA forms bundles in a crowded micro-space with F-actin even in the absence of multivalent cations.

UR - http://www.scopus.com/inward/record.url?scp=62449336412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62449336412&partnerID=8YFLogxK

U2 - 10.1109/MHS.2008.4752423

DO - 10.1109/MHS.2008.4752423

M3 - Conference contribution

AN - SCOPUS:62449336412

SN - 9781424429196

T3 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

SP - 60

EP - 64

BT - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"

ER -