Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines

Ryosuke Fujita, Ryusei Kuwata, Daisuke Kobayashi, Arlene Garcia Bertuso, Haruhiko Isawa, Kyoko Sawabe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We isolated two distinct viruses from mosquitoes collected in Bustos, Bulacan province, Philippines, in 2009. These viruses show rapid replication and strong cytopathic effects in mosquito C6/36 cells. Whole-genome analysis of these viruses demonstrated that both viruses belong to the negevirus group. One of the viruses, from Culex vishunui mosquitoes, is a new strain of Negev virus. The other virus, from a Mansonia sp. mosquito, is a new negevirus designated Bustos virus. Gene expression analysis of the Bustos virus revealed that infected cells contain viral subgenomic RNAs that probably include open reading frame (ORF) 2 or ORF3. Purified Bustos virus particles contained at least three proteins, and the major component (a probable major capsid protein) is encoded by ORF3. Bustos virus did not show infectivity in mammalian BHK-21 cells, suggesting that it is an insect-specific virus, like other known negeviruses.

Original languageEnglish
Pages (from-to)79-88
Number of pages10
JournalArchives of Virology
Volume162
Issue number1
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

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Sterculiaceae
Philippines
Culicidae
Viruses
Insect Viruses
Culex
Viral RNA
Capsid Proteins
Virion
Open Reading Frames

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines. / Fujita, Ryosuke; Kuwata, Ryusei; Kobayashi, Daisuke; Bertuso, Arlene Garcia; Isawa, Haruhiko; Sawabe, Kyoko.

In: Archives of Virology, Vol. 162, No. 1, 01.01.2017, p. 79-88.

Research output: Contribution to journalArticle

Fujita, Ryosuke ; Kuwata, Ryusei ; Kobayashi, Daisuke ; Bertuso, Arlene Garcia ; Isawa, Haruhiko ; Sawabe, Kyoko. / Bustos virus, a new member of the negevirus group isolated from a Mansonia mosquito in the Philippines. In: Archives of Virology. 2017 ; Vol. 162, No. 1. pp. 79-88.
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