Butyrate attenuates inflammation and lipolysis generated by the interaction of adipocytes and macrophages

Hideo Ohira, Yoshio Fujioka, Chikae Katagiri, Rie Mamoto, Michiko Aoyama-Ishikawa, Katsumi Amako, Yoshihiro Izumi, Shin Nishiumi, Masaru Yoshida, Makoto Usami, Masamichi Ikeda

Research output: Contribution to journalArticle

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Abstract

Aim: Paracrine interaction between macrophages and adipocytes in obese visceral fat tissues is thought to be a trigger of chronic inflammation. The immunomodulatory effect of the short chain fatty acid, butyric acid, has been demonstrated. We hypothesize that sodium butyrate (butyrate) attenuates inflammatory responses and lipolysis generated by the interaction of macrophages and adipocytes. Methods: Using contact or transwell co-culture methods with differentiated 3T3-L1 adipocytes and RAW264.7 macrophages, we investigated the effects of butyrate on the production of tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein 1 (MCP-1), interleukin 6 (IL-6), and the release of free glycerol, free fatty acids (FFAs) into the medium. We also examined the activity of nuclear factor-kappaB (NF-κB) and the phosphorylation of mitogen-activated protein kinases (MAPKs) in co-cultured macrophages, as well as lipase activity and expression in co-cultured adipocytes. Results: We found increased production of TNF-α, MCP-1, IL-6, and free glycerol, FFAs in the coculture medium, and butyrate significantly reduced them. Butyrate inhibited the phosphorylation of MAPKs, the activity of NF-κB in co-cultured macrophages, and suppressed lipase activity in co-cultured adipocytes. Lipase inhibitors significantly attenuated the production of TNF-α, MCP-1 and IL-6 in the co-culture medium as effectively as butyrate. Butyrate suppressed the protein production of adipose triglyceride lipase, hormone sensitive lipase, and fatty acid-binding protein 4 in co-cultured adipocytes. Pertussis toxin, which is known to block GPR41 completely, inhibited the antilipolysis effect of butyrate. Conclusion: Butyrate suppresses inflammatory responses generated by the interaction of adipocytes and macrophages through reduced lipolysis and inhibition of inflammatory signaling.

Original languageEnglish
Pages (from-to)425-442
Number of pages18
JournalJournal of atherosclerosis and thrombosis
Volume20
Issue number5
DOIs
Publication statusPublished - Jun 5 2013

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Butyrates
Macrophages
Lipolysis
Adipocytes
Inflammation
Lipase
Chemokine CCL2
Coculture Techniques
Interleukin-6
Phosphorylation
Butyric Acid
Tumor Necrosis Factor-alpha
Mitogen-Activated Protein Kinases
Nonesterified Fatty Acids
Glycerol
Sterol Esterase
Fatty Acid-Binding Proteins
Intra-Abdominal Fat
Volatile Fatty Acids
Pertussis Toxin

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine
  • Biochemistry, medical

Cite this

Ohira, H., Fujioka, Y., Katagiri, C., Mamoto, R., Aoyama-Ishikawa, M., Amako, K., ... Ikeda, M. (2013). Butyrate attenuates inflammation and lipolysis generated by the interaction of adipocytes and macrophages. Journal of atherosclerosis and thrombosis, 20(5), 425-442. https://doi.org/10.5551/jat.15065

Butyrate attenuates inflammation and lipolysis generated by the interaction of adipocytes and macrophages. / Ohira, Hideo; Fujioka, Yoshio; Katagiri, Chikae; Mamoto, Rie; Aoyama-Ishikawa, Michiko; Amako, Katsumi; Izumi, Yoshihiro; Nishiumi, Shin; Yoshida, Masaru; Usami, Makoto; Ikeda, Masamichi.

In: Journal of atherosclerosis and thrombosis, Vol. 20, No. 5, 05.06.2013, p. 425-442.

Research output: Contribution to journalArticle

Ohira, H, Fujioka, Y, Katagiri, C, Mamoto, R, Aoyama-Ishikawa, M, Amako, K, Izumi, Y, Nishiumi, S, Yoshida, M, Usami, M & Ikeda, M 2013, 'Butyrate attenuates inflammation and lipolysis generated by the interaction of adipocytes and macrophages', Journal of atherosclerosis and thrombosis, vol. 20, no. 5, pp. 425-442. https://doi.org/10.5551/jat.15065
Ohira, Hideo ; Fujioka, Yoshio ; Katagiri, Chikae ; Mamoto, Rie ; Aoyama-Ishikawa, Michiko ; Amako, Katsumi ; Izumi, Yoshihiro ; Nishiumi, Shin ; Yoshida, Masaru ; Usami, Makoto ; Ikeda, Masamichi. / Butyrate attenuates inflammation and lipolysis generated by the interaction of adipocytes and macrophages. In: Journal of atherosclerosis and thrombosis. 2013 ; Vol. 20, No. 5. pp. 425-442.
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AU - Ohira, Hideo

AU - Fujioka, Yoshio

AU - Katagiri, Chikae

AU - Mamoto, Rie

AU - Aoyama-Ishikawa, Michiko

AU - Amako, Katsumi

AU - Izumi, Yoshihiro

AU - Nishiumi, Shin

AU - Yoshida, Masaru

AU - Usami, Makoto

AU - Ikeda, Masamichi

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N2 - Aim: Paracrine interaction between macrophages and adipocytes in obese visceral fat tissues is thought to be a trigger of chronic inflammation. The immunomodulatory effect of the short chain fatty acid, butyric acid, has been demonstrated. We hypothesize that sodium butyrate (butyrate) attenuates inflammatory responses and lipolysis generated by the interaction of macrophages and adipocytes. Methods: Using contact or transwell co-culture methods with differentiated 3T3-L1 adipocytes and RAW264.7 macrophages, we investigated the effects of butyrate on the production of tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein 1 (MCP-1), interleukin 6 (IL-6), and the release of free glycerol, free fatty acids (FFAs) into the medium. We also examined the activity of nuclear factor-kappaB (NF-κB) and the phosphorylation of mitogen-activated protein kinases (MAPKs) in co-cultured macrophages, as well as lipase activity and expression in co-cultured adipocytes. Results: We found increased production of TNF-α, MCP-1, IL-6, and free glycerol, FFAs in the coculture medium, and butyrate significantly reduced them. Butyrate inhibited the phosphorylation of MAPKs, the activity of NF-κB in co-cultured macrophages, and suppressed lipase activity in co-cultured adipocytes. Lipase inhibitors significantly attenuated the production of TNF-α, MCP-1 and IL-6 in the co-culture medium as effectively as butyrate. Butyrate suppressed the protein production of adipose triglyceride lipase, hormone sensitive lipase, and fatty acid-binding protein 4 in co-cultured adipocytes. Pertussis toxin, which is known to block GPR41 completely, inhibited the antilipolysis effect of butyrate. Conclusion: Butyrate suppresses inflammatory responses generated by the interaction of adipocytes and macrophages through reduced lipolysis and inhibition of inflammatory signaling.

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