Bystander-killing effect and cyclic induction of TNF-α gene under heat-inducible promoter gadd 153

Akira Ito, Masashige Shinkai, Isabelle Anne Bouhon, Hiroyuki Honda, Takeshi Kobayashi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The bystander-killing effect and cyclic induction of tumor necrosis factor (TNF)-a gene expression under the heat-inducible promoter gadd 153 (growth arrest and DNA damage) were investigated. The plasmid harboring the TNF-a gene under the control of the gadd 153 promoter (pGadTNF) was introduced into cells of the human glioma cell line U251-SP. When the transfected cells were exposed to heat treatment, TNF-a gene expression was induced. The heated pGadTNF-transfected cells were co-cultured the nonheated pGadTNF-transfected cells as bystander cells. As a result, TNF-a gene expression in the bystander cells was observed. The level of TNF-a gene expression was further enhanced in the co-cultured cells. Due to this cyclic induction, a strong cytotoxic effect on the bystander cells was observed. Based on this bystander-killing effect, it was concluded that the transfection of the TNF-a gene under the control of the gadd 153 promoter into tumor cells combined with hyperthermia would be a potent tool for cancer therapy.

Original languageEnglish
Pages (from-to)437-441
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume90
Issue number4
DOIs
Publication statusPublished - Jan 1 2000

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Bystander-killing effect and cyclic induction of TNF-α gene under heat-inducible promoter gadd 153'. Together they form a unique fingerprint.

  • Cite this