C-Terminal Binding Protein (CtBP) Activates the Expression of E-Box Clock Genes with CLOCK/CYCLE in Drosophila

Taichi Q. Itoh, Akira Matsumoto, Teiichi Tanimura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In Drosophila, CLOCK/CYCLE heterodimer (CLK/CYC) is the primary activator of circadian clock genes that contain the E-box sequence in their promoter regions (hereafter referred to as "E-box clock genes"). Although extensive studies have investigated the feedback regulation of clock genes, little is known regarding other factors acting with CLK/CYC. Here we show that Drosophila C-terminal binding protein (dCtBP), a transcriptional co-factor, is involved in the regulation of the E-box clock genes. In vivo overexpression of dCtBP in clock cells lengthened or abolished circadian locomotor rhythm with up-regulation of a subset of the E-box clock genes, period (per), vrille (vri), and PAR domain protein 1ε (Pdp1ε). Co-expression of dCtBP with CLK in vitro also increased the promoter activity of per, vri, Pdp1ε and cwo depending on the amount of dCtBP expression, whereas no effect was observed without CLK. The activation of these clock genes in vitro was not observed when we used mutated dCtBP which carries amino acid substitutions in NAD+ domain. These results suggest that dCtBP generally acts as a putative co-activator of CLK/CYC through the E-box sequence.

Original languageEnglish
Article numbere63113
JournalPloS one
Volume8
Issue number4
DOIs
Publication statusPublished - Apr 30 2013

Fingerprint

Drosophila
binding proteins
Clocks
protein synthesis
Genes
E-Box Elements
genes
promoter regions
Circadian Clocks
Amino Acid Substitution
Circadian Rhythm
Genetic Promoter Regions
NAD
amino acid substitution
Transcriptional Activation
C-terminal binding protein
circadian rhythm
Up-Regulation
Proteins
Substitution reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

C-Terminal Binding Protein (CtBP) Activates the Expression of E-Box Clock Genes with CLOCK/CYCLE in Drosophila. / Itoh, Taichi Q.; Matsumoto, Akira; Tanimura, Teiichi.

In: PloS one, Vol. 8, No. 4, e63113, 30.04.2013.

Research output: Contribution to journalArticle

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