Abstract
Sexual aggregation involved in conjugative transfer of Enterococcus faecalis plasmid pADl is enhanced by the sex pheromone cADl, which is excreted from recipient cells. A membrane-anchored 137 kDa protein is a pADl-encoded aggregation substance designated asal, which is responsible for cell-cell contact and leads to the aggregation of cells. An AD74 protein is a proteolytic product corresponding to the N-terminal half of asal. The C-terminal of AD74 was identified as lysine at position 510 (K-510) by liquid chromatography/mass spectrometry (LC/MS): it indicates that asal is cleaved specifically between K-510 and G-511.
| Original language | English |
|---|---|
| Pages (from-to) | 127-131 |
| Number of pages | 5 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Volume | 56 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 1992 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
C-Terminal Identification of AD74, a Proteolytic Product of Enterococcus faecalis Aggregation Substance : Application of Liquid Chromatography/Mass Spectrometry. / Nakayama, Jiro; Watarai, Hiroshi; Isogai, Akira; Suzuki, Akinori.
In: Bioscience, Biotechnology, and Biochemistry, Vol. 56, No. 1, 01.01.1992, p. 127-131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - C-Terminal Identification of AD74, a Proteolytic Product of Enterococcus faecalis Aggregation Substance
T2 - Application of Liquid Chromatography/Mass Spectrometry
AU - Nakayama, Jiro
AU - Watarai, Hiroshi
AU - Isogai, Akira
AU - Suzuki, Akinori
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Sexual aggregation involved in conjugative transfer of Enterococcus faecalis plasmid pADl is enhanced by the sex pheromone cADl, which is excreted from recipient cells. A membrane-anchored 137 kDa protein is a pADl-encoded aggregation substance designated asal, which is responsible for cell-cell contact and leads to the aggregation of cells. An AD74 protein is a proteolytic product corresponding to the N-terminal half of asal. The C-terminal of AD74 was identified as lysine at position 510 (K-510) by liquid chromatography/mass spectrometry (LC/MS): it indicates that asal is cleaved specifically between K-510 and G-511.
AB - Sexual aggregation involved in conjugative transfer of Enterococcus faecalis plasmid pADl is enhanced by the sex pheromone cADl, which is excreted from recipient cells. A membrane-anchored 137 kDa protein is a pADl-encoded aggregation substance designated asal, which is responsible for cell-cell contact and leads to the aggregation of cells. An AD74 protein is a proteolytic product corresponding to the N-terminal half of asal. The C-terminal of AD74 was identified as lysine at position 510 (K-510) by liquid chromatography/mass spectrometry (LC/MS): it indicates that asal is cleaved specifically between K-510 and G-511.
UR - http://www.scopus.com/inward/record.url?scp=0026485964&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026485964&partnerID=8YFLogxK
U2 - 10.1271/bbb.56.127
DO - 10.1271/bbb.56.127
M3 - Article
C2 - 1368124
AN - SCOPUS:0026485964
VL - 56
SP - 127
EP - 131
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 1
ER -