C-type lectin receptor Dectin-2 binds to an endogenous protein β-glucuronidase on dendritic cells

Daiki Mori, Kensuke Shibata, Shou Yamasaki

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    C-type lectin receptors (CLRs) recognize pathogen-derived ligands and abnormal self that trigger protective immune responses. However, the precise nature of self ligands recognized by CLRs remains to be determined. Here, we found that Dectin-2 recognizes bone marrow-derived dendritic cells (BMDCs) using Dectin-2-expressing reporter cells. This activity was inhibited by an excessive amount of mannose, and by the mutation of mannose-binding motif in Dectin-2. β-glucuronidase (Gusb) was identified as a protein bound to Dectin-2 and mutations of N-glycosylation sites in Gusb impaired the binding of Gusb to Dectin-2. Overexpression of Gusb in a macrophage cell line conferred an ability to stimulate Dectin-2-expressing reporter cells. Our study suggests that a glycosylated protein with mannose-related structure is recognized by Dectin-2.

    Original languageEnglish
    Article numbere0169562
    JournalPloS one
    Volume12
    Issue number1
    DOIs
    Publication statusPublished - Jan 1 2017

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    C-Type Lectins
    Glucuronidase
    dendritic cells
    mannose
    lectins
    Dendritic Cells
    receptors
    Mannose
    mutation
    Proteins
    proteins
    glycosylation
    bone marrow
    macrophages
    cell lines
    immune response
    cells
    Ligands
    pathogens
    Glycosylation

    All Science Journal Classification (ASJC) codes

    • Medicine(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Cite this

    C-type lectin receptor Dectin-2 binds to an endogenous protein β-glucuronidase on dendritic cells. / Mori, Daiki; Shibata, Kensuke; Yamasaki, Shou.

    In: PloS one, Vol. 12, No. 1, e0169562, 01.01.2017.

    Research output: Contribution to journalArticle

    Mori, Daiki ; Shibata, Kensuke ; Yamasaki, Shou. / C-type lectin receptor Dectin-2 binds to an endogenous protein β-glucuronidase on dendritic cells. In: PloS one. 2017 ; Vol. 12, No. 1.
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