TY - JOUR
T1 -
Ca release in the endoplasmic reticulum of Guinea pig peritoneal macrophages
1
AU - Hirata, Masato
AU - Hamachi, Takafumi
AU - Hashimoto, Toshihiko
AU - Suematsu, Eiichi
AU - Koga, Toshitaka
PY - 1983/1/1
Y1 - 1983/1/1
N2 -
Passive permeability of the endoplasmic reticulum of saponin-treated macrophages to Ca
2+
was studied by the filtration method using
45
Ca. The Ca
2+
release from the endoplasmic reticulum of macrophages was enhanced by the presence of submicromolar concentrations of Ca
2+
in the medium. The Ca
2+
release was enhanced by caffeine, and suppressed by MgCl
2
. These phenomena are similar to the Ca
2+
-induced Ca
2+
release reported for the sarcoplasmic reticulum of skeletal muscle. On the other hand, adenine suppressed the Ca
2+
release from the endoplasmic reticulum, while it reportedly enhanced the Ca
2+
-induced Ca
2+
release of the skeletal muscle. The threshold concentration of Ca
2+
for the Ca
2+
-induced Ca
2+
release was approximately 10
-8
M in the presence of 0.95 mM MgCl
2
in macrophages. The spontaneous spreading of macrophages and spontaneous migration of macrophages were inhibited by adenine, and also by caffeine in spite of the enhancement of the Ca
2+
-induced Ca
2+
release.
AB -
Passive permeability of the endoplasmic reticulum of saponin-treated macrophages to Ca
2+
was studied by the filtration method using
45
Ca. The Ca
2+
release from the endoplasmic reticulum of macrophages was enhanced by the presence of submicromolar concentrations of Ca
2+
in the medium. The Ca
2+
release was enhanced by caffeine, and suppressed by MgCl
2
. These phenomena are similar to the Ca
2+
-induced Ca
2+
release reported for the sarcoplasmic reticulum of skeletal muscle. On the other hand, adenine suppressed the Ca
2+
release from the endoplasmic reticulum, while it reportedly enhanced the Ca
2+
-induced Ca
2+
release of the skeletal muscle. The threshold concentration of Ca
2+
for the Ca
2+
-induced Ca
2+
release was approximately 10
-8
M in the presence of 0.95 mM MgCl
2
in macrophages. The spontaneous spreading of macrophages and spontaneous migration of macrophages were inhibited by adenine, and also by caffeine in spite of the enhancement of the Ca
2+
-induced Ca
2+
release.
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U2 - 10.1093/oxfordjournals.jbchem.a134460
DO - 10.1093/oxfordjournals.jbchem.a134460
M3 - Article
C2 - 6654849
AN - SCOPUS:0020838175
VL - 94
SP - 1155
EP - 1163
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 4
ER -