Ca release in the endoplasmic reticulum of Guinea pig peritoneal macrophages 1

Masato Hirata, Takafumi Hamachi, Toshihiko Hashimoto, Eiichi Suematsu, Toshitaka Koga

Research output: Contribution to journalArticle

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Abstract

Passive permeability of the endoplasmic reticulum of saponin-treated macrophages to Ca 2+ was studied by the filtration method using 45 Ca. The Ca 2+ release from the endoplasmic reticulum of macrophages was enhanced by the presence of submicromolar concentrations of Ca 2+ in the medium. The Ca 2+ release was enhanced by caffeine, and suppressed by MgCl 2 . These phenomena are similar to the Ca 2+ -induced Ca 2+ release reported for the sarcoplasmic reticulum of skeletal muscle. On the other hand, adenine suppressed the Ca 2+ release from the endoplasmic reticulum, while it reportedly enhanced the Ca 2+ -induced Ca 2+ release of the skeletal muscle. The threshold concentration of Ca 2+ for the Ca 2+ -induced Ca 2+ release was approximately 10 -8 M in the presence of 0.95 mM MgCl 2 in macrophages. The spontaneous spreading of macrophages and spontaneous migration of macrophages were inhibited by adenine, and also by caffeine in spite of the enhancement of the Ca 2+ -induced Ca 2+ release.

Original languageEnglish
Pages (from-to)1155-1163
Number of pages9
JournalJournal of biochemistry
Volume94
Issue number4
DOIs
Publication statusPublished - Jan 1 1983

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Macrophages
Peritoneal Macrophages
Endoplasmic Reticulum
Guinea Pigs
Adenine
Caffeine
Muscle
Skeletal Muscle
Saponins
Sarcoplasmic Reticulum
Permeability

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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Ca release in the endoplasmic reticulum of Guinea pig peritoneal macrophages 1 . / Hirata, Masato; Hamachi, Takafumi; Hashimoto, Toshihiko; Suematsu, Eiichi; Koga, Toshitaka.

In: Journal of biochemistry, Vol. 94, No. 4, 01.01.1983, p. 1155-1163.

Research output: Contribution to journalArticle

Hirata, Masato ; Hamachi, Takafumi ; Hashimoto, Toshihiko ; Suematsu, Eiichi ; Koga, Toshitaka. / Ca release in the endoplasmic reticulum of Guinea pig peritoneal macrophages 1 In: Journal of biochemistry. 1983 ; Vol. 94, No. 4. pp. 1155-1163.
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