Abstract
Passive permeability of the endoplasmic reticulum of saponin-treated macrophages to Ca 2+ was studied by the filtration method using 45 Ca. The Ca 2+ release from the endoplasmic reticulum of macrophages was enhanced by the presence of submicromolar concentrations of Ca 2+ in the medium. The Ca 2+ release was enhanced by caffeine, and suppressed by MgCl 2 . These phenomena are similar to the Ca 2+ -induced Ca 2+ release reported for the sarcoplasmic reticulum of skeletal muscle. On the other hand, adenine suppressed the Ca 2+ release from the endoplasmic reticulum, while it reportedly enhanced the Ca 2+ -induced Ca 2+ release of the skeletal muscle. The threshold concentration of Ca 2+ for the Ca 2+ -induced Ca 2+ release was approximately 10 -8 M in the presence of 0.95 mM MgCl 2 in macrophages. The spontaneous spreading of macrophages and spontaneous migration of macrophages were inhibited by adenine, and also by caffeine in spite of the enhancement of the Ca 2+ -induced Ca 2+ release.
| Original language | English |
|---|---|
| Pages (from-to) | 1155-1163 |
| Number of pages | 9 |
| Journal | Journal of biochemistry |
| Volume | 94 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1 1983 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
Cite this
Ca release in the endoplasmic reticulum of Guinea pig peritoneal macrophages 1 . / Hirata, Masato; Hamachi, Takafumi; Hashimoto, Toshihiko; Suematsu, Eiichi; Koga, Toshitaka.
In: Journal of biochemistry, Vol. 94, No. 4, 01.01.1983, p. 1155-1163.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ca release in the endoplasmic reticulum of Guinea pig peritoneal macrophages 1
AU - Hirata, Masato
AU - Hamachi, Takafumi
AU - Hashimoto, Toshihiko
AU - Suematsu, Eiichi
AU - Koga, Toshitaka
PY - 1983/1/1
Y1 - 1983/1/1
N2 - Passive permeability of the endoplasmic reticulum of saponin-treated macrophages to Ca 2+ was studied by the filtration method using 45 Ca. The Ca 2+ release from the endoplasmic reticulum of macrophages was enhanced by the presence of submicromolar concentrations of Ca 2+ in the medium. The Ca 2+ release was enhanced by caffeine, and suppressed by MgCl 2 . These phenomena are similar to the Ca 2+ -induced Ca 2+ release reported for the sarcoplasmic reticulum of skeletal muscle. On the other hand, adenine suppressed the Ca 2+ release from the endoplasmic reticulum, while it reportedly enhanced the Ca 2+ -induced Ca 2+ release of the skeletal muscle. The threshold concentration of Ca 2+ for the Ca 2+ -induced Ca 2+ release was approximately 10 -8 M in the presence of 0.95 mM MgCl 2 in macrophages. The spontaneous spreading of macrophages and spontaneous migration of macrophages were inhibited by adenine, and also by caffeine in spite of the enhancement of the Ca 2+ -induced Ca 2+ release.
AB - Passive permeability of the endoplasmic reticulum of saponin-treated macrophages to Ca 2+ was studied by the filtration method using 45 Ca. The Ca 2+ release from the endoplasmic reticulum of macrophages was enhanced by the presence of submicromolar concentrations of Ca 2+ in the medium. The Ca 2+ release was enhanced by caffeine, and suppressed by MgCl 2 . These phenomena are similar to the Ca 2+ -induced Ca 2+ release reported for the sarcoplasmic reticulum of skeletal muscle. On the other hand, adenine suppressed the Ca 2+ release from the endoplasmic reticulum, while it reportedly enhanced the Ca 2+ -induced Ca 2+ release of the skeletal muscle. The threshold concentration of Ca 2+ for the Ca 2+ -induced Ca 2+ release was approximately 10 -8 M in the presence of 0.95 mM MgCl 2 in macrophages. The spontaneous spreading of macrophages and spontaneous migration of macrophages were inhibited by adenine, and also by caffeine in spite of the enhancement of the Ca 2+ -induced Ca 2+ release.
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U2 - 10.1093/oxfordjournals.jbchem.a134460
DO - 10.1093/oxfordjournals.jbchem.a134460
M3 - Article
VL - 94
SP - 1155
EP - 1163
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 4
ER -