Calcium-dependent hyperexcitability of hippocampal CA1 pyramidal cells in an in vitro slice after ethanol withdrawal of the rat

Tomomi Shindou, Shigenori Watanabe, Osamu Kamata, Kenji Yamamoto, Hiroshi Nakanishi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The physiological profiles contributing to hyperexcitability of hippocampal CA1 neurons following ethanol withdrawal (EW) were examined in an in vitro slice preparation obtained from EW rats. Sixty-two percent of CA1 neurons in slices from EW rats exhibited intrinsic burst property which was rarely observed in those from control animals. The mean duration of plateau component of calcium (Ca) spikes was significantly increased after EW. The burst response evoked by either synaptic or direct stimulation in hippocampal CA1 neurons from EW rats was markedly depressed by high Mg solution but not by flunarizine. Furthermore, 6-cyano-7-nitroquinoxaline-2,3-dione, a non-NMDA receptor antagonist, markedly depressed the synaptically evoked burst response, while [(±)-2-carboxypiperazine-4-yl-]-propyl-1-phosphonic acid, a selective N-methyl-d-aspartate (NMDA) receptor antagonist, slightly delayed the onset of the response. The results indicate that an increase in the number of bursting hippocampal CA1 neurons associated with an augmentation of the plateau component of Ca spike contributes to the genesis of hyperexcitability in EW rats. Furthermore, non-NMDA receptor-mediated EPSP is mainly responsible for a synaptic induction of the burst response. These results are consistent with the involvement of high-threshold Ca channels in EW hyperexcitability.

Original languageEnglish
Pages (from-to)432-436
Number of pages5
JournalBrain Research
Volume656
Issue number2
DOIs
Publication statusPublished - Sep 12 1994

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Pyramidal Cells
Ethanol
Calcium
Neurons
Calcium Signaling
Flunarizine
6-Cyano-7-nitroquinoxaline-2,3-dione
Excitatory Postsynaptic Potentials
Calcium Channels
In Vitro Techniques
aspartic acid receptor

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Calcium-dependent hyperexcitability of hippocampal CA1 pyramidal cells in an in vitro slice after ethanol withdrawal of the rat. / Shindou, Tomomi; Watanabe, Shigenori; Kamata, Osamu; Yamamoto, Kenji; Nakanishi, Hiroshi.

In: Brain Research, Vol. 656, No. 2, 12.09.1994, p. 432-436.

Research output: Contribution to journalArticle

Shindou, Tomomi ; Watanabe, Shigenori ; Kamata, Osamu ; Yamamoto, Kenji ; Nakanishi, Hiroshi. / Calcium-dependent hyperexcitability of hippocampal CA1 pyramidal cells in an in vitro slice after ethanol withdrawal of the rat. In: Brain Research. 1994 ; Vol. 656, No. 2. pp. 432-436.
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