Calcium-induced fragmentation of skeletal muscle nebulin filaments

Ryuichi Tatsumi, Koui Takahashi

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Abstract

When chicken breast muscle myofibrils were treated with a solution containing 0.1 mM CaCl2 and 30 μg of leupeptin/ml, nebulin filaments were fragmented into 200-, 180-, 40-, 33-, and 23-kDa subfragments. All the subfragments except the 180-kDa one were released from the myofibrils. The fragmentation of nebulin filaments seems to be induced by the binding of large amounts of calcium ions. Similar changes took place in nebulin filaments in post-mortem skeletal muscle. It has been proposed that nebulin co-exists with thin (actin) filaments and participates in stabilizing their organization [Wang, K & Wright, J. (1988) J. Cell Biol. 107, 2199-2212]. Thus, the above result suggests that Ca-induced fragmentation of nebulin filaments destabilizes the organization of thin filaments and is a key factor in meat tenderization during post-rigor aging.

Original languageEnglish
Pages (from-to)775-779
Number of pages5
JournalJournal of Biochemistry
Volume112
Issue number6
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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