TY - JOUR
T1 - Calcium-induced splitting of connectin filaments into β-connectin and a 1,200-kDa subfragment
AU - Takahashi, Koui
AU - Hattori, Akihito
AU - Tatsumi, Ryuichi
AU - Takai, Kouji
PY - 1992/1/1
Y1 - 1992/1/1
N2 - When rabbit skeletal muscle myofibrils were treated with a solution containing 0.1 mM Ca2+ and 30 μg of leupeptin/ml, α-connectin, which forms very thin filaments in myofibrils, was split into β-connectin and a 1,200-kDa subfragment. A part of β-connectin located near the junction between β-connectin and the subfragment seems to have an affinity for calcium ions and to be susceptible to the binding of large amounts of calcium ions. The calcium- binding site on β-connectin is localized near the N2 line in the I band, and the subfragment Is localized adjacent to the Z disk. It is possible that connectin filaments change their elasticity during the contraction-relaxation cycle of skeletal muscle at the physiological concentration of calcium ions. Because postmortem skeletal muscles lose their elasticity and become plastic in association with the calcium-specific splitting of connectin filaments, the splitting is considered to be a factor in meat tenderization during postrigor ageing.
AB - When rabbit skeletal muscle myofibrils were treated with a solution containing 0.1 mM Ca2+ and 30 μg of leupeptin/ml, α-connectin, which forms very thin filaments in myofibrils, was split into β-connectin and a 1,200-kDa subfragment. A part of β-connectin located near the junction between β-connectin and the subfragment seems to have an affinity for calcium ions and to be susceptible to the binding of large amounts of calcium ions. The calcium- binding site on β-connectin is localized near the N2 line in the I band, and the subfragment Is localized adjacent to the Z disk. It is possible that connectin filaments change their elasticity during the contraction-relaxation cycle of skeletal muscle at the physiological concentration of calcium ions. Because postmortem skeletal muscles lose their elasticity and become plastic in association with the calcium-specific splitting of connectin filaments, the splitting is considered to be a factor in meat tenderization during postrigor ageing.
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U2 - 10.1093/oxfordjournals.jbchem.a123835
DO - 10.1093/oxfordjournals.jbchem.a123835
M3 - Article
C2 - 1500418
AN - SCOPUS:0026690389
VL - 111
SP - 778
EP - 782
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 6
ER -