Calcium ions stabilize a protein structure of hemolytic lectin CEL-III from marine invertebrate Cucumaria echinata

Imre Sallay, Sumiki Tojo, Koji Nomiyama, Yoshiaki Kouzuma, Makoto Kimura, Nobuyuki Yamasaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

CEL-III, a galactose/N-acetylgatactosamine (Gal/ GalNAc)-specific lectin purified from a marine invertebrate, Cucumaria echinata, has a strong hemolytic activity, especially toward human and rabbit erythrocytes in the presence of Ca2+. We evaluated the role of Ca2+ in hemagglutinating and hemolytic activities of CEL-III. We found that Ca2+ is closely associated with both activities of CEL-III. The fluorescence spectra of CEL-III upon binding to Ca2+ were measured. The result showed a structural change of CEL-III in the presence of Ca2+. The structural change of CEL-UI upon Ca2+ binding was further demonstrated by stabilization against urea denaturation and by insusceptibility to protease digestions. CEL-III was completely unfolded at a low concentration of 2 M urea, while CEL-III complexed with Ca2+ was stable in 6 M urea. As for protease digestions, CEL-III monomer and oligomer were readily digested by trypsin. chymotrypsin, and papain in the absence of Ca2+, while they were insusceptible to the three proteases in the presence of Ca2+. The papain digestion of the decalcified oligomer produced a large C-terminal peptide, suggestting that the C-terminal region of CEL-III may participate in oligomerization of CEL-III as a core domain.

Original languageEnglish
Pages (from-to)1347-1352
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number6
DOIs
Publication statusPublished - Jun 1 2001

Fingerprint

Cucumaria
Invertebrates
Urea
Digestion
Peptide Hydrolases
Papain
Ions
Calcium
Oligomers
Oligomerization
Proteins
Denaturation
Chymotrypsin
Galactose
Lectins
Trypsin
Stabilization
Monomers
Erythrocytes
Fluorescence

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Calcium ions stabilize a protein structure of hemolytic lectin CEL-III from marine invertebrate Cucumaria echinata. / Sallay, Imre; Tojo, Sumiki; Nomiyama, Koji; Kouzuma, Yoshiaki; Kimura, Makoto; Yamasaki, Nobuyuki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 6, 01.06.2001, p. 1347-1352.

Research output: Contribution to journalArticle

Sallay, Imre ; Tojo, Sumiki ; Nomiyama, Koji ; Kouzuma, Yoshiaki ; Kimura, Makoto ; Yamasaki, Nobuyuki. / Calcium ions stabilize a protein structure of hemolytic lectin CEL-III from marine invertebrate Cucumaria echinata. In: Bioscience, Biotechnology and Biochemistry. 2001 ; Vol. 65, No. 6. pp. 1347-1352.
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