Callus induction and plantlet regeneration in ornamental Alocasia micholitziana

Nguyen Thi Phuong Thao, Yukio Ozaki, Hiroshi Okubo

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Callus induction from petiole explants has been achieved in Alocasia micholitziana 'Green Velvet'. The highest percentage (71%) of explants inducing callus was obtained on MS medium supplemented with 0.5 μM 2,4-D and 0.5 μM kinetin in the dark after 4 months of culture. Shoots were regenerated at the highest frequency of 33.3% under light condition when 0.5 μM BA was added to MS medium with the average of 7.8±2.3 shoots per callus explant. The callus-derived shoots rooted on hormone free MS medium and within 4 weeks the plantlets were ready for acclimatization. The regenerated plants appeared morphologically similar to mother plants.

Original languageEnglish
Pages (from-to)285-289
Number of pages5
JournalPlant Cell, Tissue and Organ Culture
Volume73
Issue number3
DOIs
Publication statusPublished - Jun 1 2003

All Science Journal Classification (ASJC) codes

  • Horticulture

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