Calponin and h-caldesmon expression in atypical fibroxanthoma and superficial leiomyosarcoma

A. Sakamoto, Yoshinao Oda, Hidetaka Yamamoto, Y. Oshiro, K. Miyajima, E. Itakura, S. Tamiya, Y. Honda, A. Ishihara, Y. Iwamoto, M. Tsuneyoshi

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

To evaluate smooth muscle differentiation, myogenic markers [desmin, alpha-smooth muscle actin (SMA), and muscle-specific actin (HHF35)] have been widely used. Calponin and h-caldesmon, which are cytoskeleton-associated actin-binding proteins, have been reported to be more specific myogenic markers, especially since myofibroblasts express a small amount of h-caldesmon. Atypical fibroxanthoma (AFX) occurs in the sun-exposed skin of the elderly and follows a benign clinical course. Histologically, AFX, which is a pleomorphic spindle cell tumor and considered to be a superficial variant of malignant fibrous histiocytoma, also mimics leiomyosarcoma. AFX has been thought to differentiate along pathways with fibrohistiocytic and myofibroblastic phenotypes. AFX (n=10), superficial leiomyosarcoma (S-LMS) (n=17) and benign fibrous histiocytoma (BFH) (n=17) were analyzed for myofibroblastic and smooth muscle differentiation immunohistochemically from the viewpoint of comparison. AFX and BFH showed immunoreactivities respectively for calponin (3/10, 11/17), desmin (3/10, 1/17), SMA (3/10, 13/17), and HHF35 (1/10, 5/17), but failed to express h-caldesmon (0/10, 0/17). S-LMS had a high immunoreactive rate of calponin (17/17), desmin (13/17), SMA (16/17), and HHF35 (16/17), while also expressing caldesmon (11/17). The results reveal that AFX and BFH have immunoreactivities for several myogenic markers, with myofibroblastic differentiation (calponin: ±, h-caldesmon: -), but without the smooth muscle differentiation seen in S-LMS (calponin:+, h-caldesmon: ±). In addition, calponin and h-caldesmon are considered to be useful markers for distinguishing AFX from S-LMS.

Original languageEnglish
Pages (from-to)404-409
Number of pages6
JournalVirchows Archiv
Volume440
Issue number4
DOIs
Publication statusPublished - Apr 27 2002

Fingerprint

Calmodulin-Binding Proteins
Leiomyosarcoma
Smooth Muscle
Benign Fibrous Histiocytoma
Desmin
Actins
Microfilament Proteins
Malignant Fibrous Histiocytoma
Myofibroblasts
Differentiation Antigens
Solar System
calponin
Cytoskeleton
Phenotype
Muscles
Skin

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Calponin and h-caldesmon expression in atypical fibroxanthoma and superficial leiomyosarcoma. / Sakamoto, A.; Oda, Yoshinao; Yamamoto, Hidetaka; Oshiro, Y.; Miyajima, K.; Itakura, E.; Tamiya, S.; Honda, Y.; Ishihara, A.; Iwamoto, Y.; Tsuneyoshi, M.

In: Virchows Archiv, Vol. 440, No. 4, 27.04.2002, p. 404-409.

Research output: Contribution to journalArticle

Sakamoto, A, Oda, Y, Yamamoto, H, Oshiro, Y, Miyajima, K, Itakura, E, Tamiya, S, Honda, Y, Ishihara, A, Iwamoto, Y & Tsuneyoshi, M 2002, 'Calponin and h-caldesmon expression in atypical fibroxanthoma and superficial leiomyosarcoma', Virchows Archiv, vol. 440, no. 4, pp. 404-409. https://doi.org/10.1007/s004280100521
Sakamoto, A. ; Oda, Yoshinao ; Yamamoto, Hidetaka ; Oshiro, Y. ; Miyajima, K. ; Itakura, E. ; Tamiya, S. ; Honda, Y. ; Ishihara, A. ; Iwamoto, Y. ; Tsuneyoshi, M. / Calponin and h-caldesmon expression in atypical fibroxanthoma and superficial leiomyosarcoma. In: Virchows Archiv. 2002 ; Vol. 440, No. 4. pp. 404-409.
@article{b6ad5a5f703443deb310a4ead169cbcc,
title = "Calponin and h-caldesmon expression in atypical fibroxanthoma and superficial leiomyosarcoma",
abstract = "To evaluate smooth muscle differentiation, myogenic markers [desmin, alpha-smooth muscle actin (SMA), and muscle-specific actin (HHF35)] have been widely used. Calponin and h-caldesmon, which are cytoskeleton-associated actin-binding proteins, have been reported to be more specific myogenic markers, especially since myofibroblasts express a small amount of h-caldesmon. Atypical fibroxanthoma (AFX) occurs in the sun-exposed skin of the elderly and follows a benign clinical course. Histologically, AFX, which is a pleomorphic spindle cell tumor and considered to be a superficial variant of malignant fibrous histiocytoma, also mimics leiomyosarcoma. AFX has been thought to differentiate along pathways with fibrohistiocytic and myofibroblastic phenotypes. AFX (n=10), superficial leiomyosarcoma (S-LMS) (n=17) and benign fibrous histiocytoma (BFH) (n=17) were analyzed for myofibroblastic and smooth muscle differentiation immunohistochemically from the viewpoint of comparison. AFX and BFH showed immunoreactivities respectively for calponin (3/10, 11/17), desmin (3/10, 1/17), SMA (3/10, 13/17), and HHF35 (1/10, 5/17), but failed to express h-caldesmon (0/10, 0/17). S-LMS had a high immunoreactive rate of calponin (17/17), desmin (13/17), SMA (16/17), and HHF35 (16/17), while also expressing caldesmon (11/17). The results reveal that AFX and BFH have immunoreactivities for several myogenic markers, with myofibroblastic differentiation (calponin: ±, h-caldesmon: -), but without the smooth muscle differentiation seen in S-LMS (calponin:+, h-caldesmon: ±). In addition, calponin and h-caldesmon are considered to be useful markers for distinguishing AFX from S-LMS.",
author = "A. Sakamoto and Yoshinao Oda and Hidetaka Yamamoto and Y. Oshiro and K. Miyajima and E. Itakura and S. Tamiya and Y. Honda and A. Ishihara and Y. Iwamoto and M. Tsuneyoshi",
year = "2002",
month = "4",
day = "27",
doi = "10.1007/s004280100521",
language = "English",
volume = "440",
pages = "404--409",
journal = "Virchows Archiv",
issn = "0945-6317",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Calponin and h-caldesmon expression in atypical fibroxanthoma and superficial leiomyosarcoma

AU - Sakamoto, A.

AU - Oda, Yoshinao

AU - Yamamoto, Hidetaka

AU - Oshiro, Y.

AU - Miyajima, K.

AU - Itakura, E.

AU - Tamiya, S.

AU - Honda, Y.

AU - Ishihara, A.

AU - Iwamoto, Y.

AU - Tsuneyoshi, M.

PY - 2002/4/27

Y1 - 2002/4/27

N2 - To evaluate smooth muscle differentiation, myogenic markers [desmin, alpha-smooth muscle actin (SMA), and muscle-specific actin (HHF35)] have been widely used. Calponin and h-caldesmon, which are cytoskeleton-associated actin-binding proteins, have been reported to be more specific myogenic markers, especially since myofibroblasts express a small amount of h-caldesmon. Atypical fibroxanthoma (AFX) occurs in the sun-exposed skin of the elderly and follows a benign clinical course. Histologically, AFX, which is a pleomorphic spindle cell tumor and considered to be a superficial variant of malignant fibrous histiocytoma, also mimics leiomyosarcoma. AFX has been thought to differentiate along pathways with fibrohistiocytic and myofibroblastic phenotypes. AFX (n=10), superficial leiomyosarcoma (S-LMS) (n=17) and benign fibrous histiocytoma (BFH) (n=17) were analyzed for myofibroblastic and smooth muscle differentiation immunohistochemically from the viewpoint of comparison. AFX and BFH showed immunoreactivities respectively for calponin (3/10, 11/17), desmin (3/10, 1/17), SMA (3/10, 13/17), and HHF35 (1/10, 5/17), but failed to express h-caldesmon (0/10, 0/17). S-LMS had a high immunoreactive rate of calponin (17/17), desmin (13/17), SMA (16/17), and HHF35 (16/17), while also expressing caldesmon (11/17). The results reveal that AFX and BFH have immunoreactivities for several myogenic markers, with myofibroblastic differentiation (calponin: ±, h-caldesmon: -), but without the smooth muscle differentiation seen in S-LMS (calponin:+, h-caldesmon: ±). In addition, calponin and h-caldesmon are considered to be useful markers for distinguishing AFX from S-LMS.

AB - To evaluate smooth muscle differentiation, myogenic markers [desmin, alpha-smooth muscle actin (SMA), and muscle-specific actin (HHF35)] have been widely used. Calponin and h-caldesmon, which are cytoskeleton-associated actin-binding proteins, have been reported to be more specific myogenic markers, especially since myofibroblasts express a small amount of h-caldesmon. Atypical fibroxanthoma (AFX) occurs in the sun-exposed skin of the elderly and follows a benign clinical course. Histologically, AFX, which is a pleomorphic spindle cell tumor and considered to be a superficial variant of malignant fibrous histiocytoma, also mimics leiomyosarcoma. AFX has been thought to differentiate along pathways with fibrohistiocytic and myofibroblastic phenotypes. AFX (n=10), superficial leiomyosarcoma (S-LMS) (n=17) and benign fibrous histiocytoma (BFH) (n=17) were analyzed for myofibroblastic and smooth muscle differentiation immunohistochemically from the viewpoint of comparison. AFX and BFH showed immunoreactivities respectively for calponin (3/10, 11/17), desmin (3/10, 1/17), SMA (3/10, 13/17), and HHF35 (1/10, 5/17), but failed to express h-caldesmon (0/10, 0/17). S-LMS had a high immunoreactive rate of calponin (17/17), desmin (13/17), SMA (16/17), and HHF35 (16/17), while also expressing caldesmon (11/17). The results reveal that AFX and BFH have immunoreactivities for several myogenic markers, with myofibroblastic differentiation (calponin: ±, h-caldesmon: -), but without the smooth muscle differentiation seen in S-LMS (calponin:+, h-caldesmon: ±). In addition, calponin and h-caldesmon are considered to be useful markers for distinguishing AFX from S-LMS.

UR - http://www.scopus.com/inward/record.url?scp=0036216633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036216633&partnerID=8YFLogxK

U2 - 10.1007/s004280100521

DO - 10.1007/s004280100521

M3 - Article

VL - 440

SP - 404

EP - 409

JO - Virchows Archiv

JF - Virchows Archiv

SN - 0945-6317

IS - 4

ER -