Cancer-specific gene carriers responding to cancer microenvironment: Acidosis and hyper-activated protein kinases

Satoshi Kushio, Akira Tsuchiya, Yuta Nakamura, Takanobu Nobori, Chan Woo Kim, Guo Xi Zhao, Taiki Funamoto, Eun Kyung Lee, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Protein kinase (PK)-responsive gene carriers modified with polyethylene glycol (PEG) chains using an acid-labile linker were developed. These carriers were obtained by modifying the PEG chains and substrate peptides for the PKs (PKA or PKCα) on the branched polyethyleneimine main chain. Polyplexes formed from these carriers and plasmid DNA (pDNA) were stably dispersed under neutral pH medium. The polyplexes were also taken up by cells on the release of the PEG chains under the slightly acidic extracellular pH associated with cancer cells. The polyplexes taken up by cells resulted in gene expression when the substrate peptides were phosphorylated by the intracellular PKs to release pDNA from the polyplexes. These novel gene carriers are expected to be promising for cancer-specific gene therapy via intravenous administration.

Original languageEnglish
Article number1340005
JournalBiomedical Engineering - Applications, Basis and Communications
Volume25
Issue number5
DOIs
Publication statusPublished - Oct 1 2013

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomedical Engineering

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