Candida albicans growth on thermal cycled materials for maxillofacial prostheses in vitro

H. Nikawa, C. Jin, T. Hamada, S. Makihira, G. Polyzois

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In the present study, the growth of a single isolate of Candida albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4-70°C for 1 min, respectively; 0, 1000 and 10 000 times) 15 commercial maxillofacial materials was investigated, by monitoring pH changes in growth media. The inhibitory effect of the tissue conditioners on fungal growth was analysed using three parameters viz: (i) delay in the onset of the rapid decline in pH (ii) reduction in the rate of pH change and (iii) the pH minima reached. In the case of control materials (non-thermocycled and uncoated), significant antifungal effect was observed with two products. However, the antifungal effect of the materials was significantly reduced both by thermal cycling (Analysis of covariance [ANOVA]; P < 0.01) and a layer of protein coating (saliva, P < 0.05; serum, P < 0.01). When the interrelation between three parameters of fungal growth and the surface hydrophobicity of the materials were analysed, minimum pH of fungal growth on 10 000-thermocycled materials correlated well with the contact angles of the materials (Student t-test, P < 0.01), suggesting that thermocycling process reduced the unpolymerized components of the materials which showed the antifungal effects, resulted in that the cell growth depends on the surface hydrophobicity of the specimens. These results, taken together, suggest that the ageing of the materials and the biological fluids of the host enhanced the fungal growth on maxillofacial materials.

Original languageEnglish
Pages (from-to)755-765
Number of pages11
JournalJournal of oral rehabilitation
Volume28
Issue number8
DOIs
Publication statusPublished - Aug 2001

Fingerprint

Maxillofacial Prosthesis
Candida albicans
Hot Temperature
Growth
Hydrophobic and Hydrophilic Interactions
Saliva
In Vitro Techniques
Serum
Proteins
Students

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Candida albicans growth on thermal cycled materials for maxillofacial prostheses in vitro. / Nikawa, H.; Jin, C.; Hamada, T.; Makihira, S.; Polyzois, G.

In: Journal of oral rehabilitation, Vol. 28, No. 8, 08.2001, p. 755-765.

Research output: Contribution to journalArticle

Nikawa, H. ; Jin, C. ; Hamada, T. ; Makihira, S. ; Polyzois, G. / Candida albicans growth on thermal cycled materials for maxillofacial prostheses in vitro. In: Journal of oral rehabilitation. 2001 ; Vol. 28, No. 8. pp. 755-765.
@article{310c7c0b9f32468095611a66c47193e2,
title = "Candida albicans growth on thermal cycled materials for maxillofacial prostheses in vitro",
abstract = "In the present study, the growth of a single isolate of Candida albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4-70°C for 1 min, respectively; 0, 1000 and 10 000 times) 15 commercial maxillofacial materials was investigated, by monitoring pH changes in growth media. The inhibitory effect of the tissue conditioners on fungal growth was analysed using three parameters viz: (i) delay in the onset of the rapid decline in pH (ii) reduction in the rate of pH change and (iii) the pH minima reached. In the case of control materials (non-thermocycled and uncoated), significant antifungal effect was observed with two products. However, the antifungal effect of the materials was significantly reduced both by thermal cycling (Analysis of covariance [ANOVA]; P < 0.01) and a layer of protein coating (saliva, P < 0.05; serum, P < 0.01). When the interrelation between three parameters of fungal growth and the surface hydrophobicity of the materials were analysed, minimum pH of fungal growth on 10 000-thermocycled materials correlated well with the contact angles of the materials (Student t-test, P < 0.01), suggesting that thermocycling process reduced the unpolymerized components of the materials which showed the antifungal effects, resulted in that the cell growth depends on the surface hydrophobicity of the specimens. These results, taken together, suggest that the ageing of the materials and the biological fluids of the host enhanced the fungal growth on maxillofacial materials.",
author = "H. Nikawa and C. Jin and T. Hamada and S. Makihira and G. Polyzois",
year = "2001",
month = "8",
doi = "10.1046/j.1365-2842.2001.00706.x",
language = "English",
volume = "28",
pages = "755--765",
journal = "Journal of Oral Rehabilitation",
issn = "0305-182X",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Candida albicans growth on thermal cycled materials for maxillofacial prostheses in vitro

AU - Nikawa, H.

AU - Jin, C.

AU - Hamada, T.

AU - Makihira, S.

AU - Polyzois, G.

PY - 2001/8

Y1 - 2001/8

N2 - In the present study, the growth of a single isolate of Candida albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4-70°C for 1 min, respectively; 0, 1000 and 10 000 times) 15 commercial maxillofacial materials was investigated, by monitoring pH changes in growth media. The inhibitory effect of the tissue conditioners on fungal growth was analysed using three parameters viz: (i) delay in the onset of the rapid decline in pH (ii) reduction in the rate of pH change and (iii) the pH minima reached. In the case of control materials (non-thermocycled and uncoated), significant antifungal effect was observed with two products. However, the antifungal effect of the materials was significantly reduced both by thermal cycling (Analysis of covariance [ANOVA]; P < 0.01) and a layer of protein coating (saliva, P < 0.05; serum, P < 0.01). When the interrelation between three parameters of fungal growth and the surface hydrophobicity of the materials were analysed, minimum pH of fungal growth on 10 000-thermocycled materials correlated well with the contact angles of the materials (Student t-test, P < 0.01), suggesting that thermocycling process reduced the unpolymerized components of the materials which showed the antifungal effects, resulted in that the cell growth depends on the surface hydrophobicity of the specimens. These results, taken together, suggest that the ageing of the materials and the biological fluids of the host enhanced the fungal growth on maxillofacial materials.

AB - In the present study, the growth of a single isolate of Candida albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4-70°C for 1 min, respectively; 0, 1000 and 10 000 times) 15 commercial maxillofacial materials was investigated, by monitoring pH changes in growth media. The inhibitory effect of the tissue conditioners on fungal growth was analysed using three parameters viz: (i) delay in the onset of the rapid decline in pH (ii) reduction in the rate of pH change and (iii) the pH minima reached. In the case of control materials (non-thermocycled and uncoated), significant antifungal effect was observed with two products. However, the antifungal effect of the materials was significantly reduced both by thermal cycling (Analysis of covariance [ANOVA]; P < 0.01) and a layer of protein coating (saliva, P < 0.05; serum, P < 0.01). When the interrelation between three parameters of fungal growth and the surface hydrophobicity of the materials were analysed, minimum pH of fungal growth on 10 000-thermocycled materials correlated well with the contact angles of the materials (Student t-test, P < 0.01), suggesting that thermocycling process reduced the unpolymerized components of the materials which showed the antifungal effects, resulted in that the cell growth depends on the surface hydrophobicity of the specimens. These results, taken together, suggest that the ageing of the materials and the biological fluids of the host enhanced the fungal growth on maxillofacial materials.

UR - http://www.scopus.com/inward/record.url?scp=0035433532&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035433532&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2842.2001.00706.x

DO - 10.1046/j.1365-2842.2001.00706.x

M3 - Article

C2 - 11556957

AN - SCOPUS:0035433532

VL - 28

SP - 755

EP - 765

JO - Journal of Oral Rehabilitation

JF - Journal of Oral Rehabilitation

SN - 0305-182X

IS - 8

ER -