Candidatus Curculioniphilus buchneri,quot; a novel clade of bacterial endocellular symbionts from weevils of the genus Curculio

Hirokazu Toju, Takahiro Hosokawa, Ryuichi Koga, Naruo Nikoh, Xian Ying Meng, Nobutada Kimura, Takema Fukatsu

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Here we investigated the bacterial endosymbionts of weevils of the genus Curculio. From all four species of Curculio weevils examined, a novel group of bacterial gene sequences were consistently identified. Molecular phylogenetic analyses demonstrated that the sequences formed a distinct clade in the Gammaproteobacteria, which was not related to previously known groups of weevil endosymbionts such as Nardonella spp. and Sodalis-allied symbionts. In situ hybridization revealed that the bacterium was intracellularly harbored in a bacteriome associated with larval midgut. In adult females, the bacterium was localized in the germalia at the tip of each overiole, suggesting vertical transmission via ovarial passage. Diagnostic PCR surveys detected high prevalence of the bacterial infection in natural host populations. Electron microscopy identified the reduced cell wall of the bacterial cells, and the bacterial genes exhibited AT-biased nucleotide composition and accelerated molecular evolution, which are suggestive of a long-lasting endosymbiotic association. On the basis of these results, we conclude that the novel endocellular bacteria represent the primary symbiont of Curculio weevils and proposed the designation quot;Condidatus Curculioniphilus buchneri.quot; In addition to quot;Ca. CurcuIioniphilus,quot; we identified Sodalis-allied gammaproteobacterial endosymbionts from the chestnut weevil, Curculio sikkimensis, which exhibited partial infection frequencies in host insect populations and neither AT-biased nucleotide composition nor accelerated molecular evolution. We suggest that such Sodalis-allied secondary symbionts in weevils might provide a potential source for symbiont replacements, as has occurred in an ancestor of Sitophilus grain weevils.

Original languageEnglish
Pages (from-to)275-282
Number of pages8
JournalApplied and environmental microbiology
Volume76
Issue number1
DOIs
Publication statusPublished - Jan 1 2010

Fingerprint

Curculio
Weevils
symbiont
symbionts
Curculionidae
endosymbiont
Sodalis
endosymbionts
Enterobacteriaceae
bacterium
Bacterial Genes
Molecular Evolution
vertical transmission
gene
Bacteria
bacteria
Curculio elephas
electron microscopy
nucleotides
ancestry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Candidatus Curculioniphilus buchneri,quot; a novel clade of bacterial endocellular symbionts from weevils of the genus Curculio. / Toju, Hirokazu; Hosokawa, Takahiro; Koga, Ryuichi; Nikoh, Naruo; Meng, Xian Ying; Kimura, Nobutada; Fukatsu, Takema.

In: Applied and environmental microbiology, Vol. 76, No. 1, 01.01.2010, p. 275-282.

Research output: Contribution to journalArticle

Toju, Hirokazu ; Hosokawa, Takahiro ; Koga, Ryuichi ; Nikoh, Naruo ; Meng, Xian Ying ; Kimura, Nobutada ; Fukatsu, Takema. / Candidatus Curculioniphilus buchneri,quot; a novel clade of bacterial endocellular symbionts from weevils of the genus Curculio. In: Applied and environmental microbiology. 2010 ; Vol. 76, No. 1. pp. 275-282.
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