Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa

Futoshi Taura, Supaart Sirikantaramas, Yoshinari Shoyama, Kazuyoshi Yoshikai, Yukihiro Shoyama, Satoshi Morimoto

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.

Original languageEnglish
Pages (from-to)2929-2934
Number of pages6
JournalFEBS Letters
Volume581
Issue number16
DOIs
Publication statusPublished - Jun 26 2007

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Cannabis
Cannabinoids
Fibers
Enzymes
Acids
Polymerase chain reaction
Cyclization
Biosynthesis
Transcription
Reverse Transcription
Oxidoreductases
Complementary DNA
Genes
Polymerase Chain Reaction
cannabidiolic acid synthase
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa. / Taura, Futoshi; Sirikantaramas, Supaart; Shoyama, Yoshinari; Yoshikai, Kazuyoshi; Shoyama, Yukihiro; Morimoto, Satoshi.

In: FEBS Letters, Vol. 581, No. 16, 26.06.2007, p. 2929-2934.

Research output: Contribution to journalArticle

Taura, Futoshi ; Sirikantaramas, Supaart ; Shoyama, Yoshinari ; Yoshikai, Kazuyoshi ; Shoyama, Yukihiro ; Morimoto, Satoshi. / Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa. In: FEBS Letters. 2007 ; Vol. 581, No. 16. pp. 2929-2934.
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