Abstract
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.
| Original language | English |
|---|---|
| Pages (from-to) | 2929-2934 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 581 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - Jun 26 2007 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa. / Taura, Futoshi; Sirikantaramas, Supaart; Shoyama, Yoshinari; Yoshikai, Kazuyoshi; Shoyama, Yukihiro; Morimoto, Satoshi.
In: FEBS Letters, Vol. 581, No. 16, 26.06.2007, p. 2929-2934.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa
AU - Taura, Futoshi
AU - Sirikantaramas, Supaart
AU - Shoyama, Yoshinari
AU - Yoshikai, Kazuyoshi
AU - Shoyama, Yukihiro
AU - Morimoto, Satoshi
PY - 2007/6/26
Y1 - 2007/6/26
N2 - Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.
AB - Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.
UR - http://www.scopus.com/inward/record.url?scp=34250179866&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250179866&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2007.05.043
DO - 10.1016/j.febslet.2007.05.043
M3 - Article
C2 - 17544411
AN - SCOPUS:34250179866
VL - 581
SP - 2929
EP - 2934
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 16
ER -