Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa

Futoshi Taura; Supaart Sirikantaramas; Yoshinari Shoyama; Kazuyoshi Yoshikai; Yukihiro Shoyama; Satoshi Morimoto

doi:10.1016/j.febslet.2007.05.043

Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa

Futoshi Taura, Supaart Sirikantaramas, Yoshinari Shoyama, Kazuyoshi Yoshikai, Yukihiro Shoyama, Satoshi Morimoto

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

100 Citations (Scopus)

Abstract

Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.

Original language	English
Pages (from-to)	2929-2934
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	16
DOIs	https://doi.org/10.1016/j.febslet.2007.05.043
Publication status	Published - Jun 26 2007

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa",

abstract = "Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.",

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AU - Taura, Futoshi

AU - Sirikantaramas, Supaart

AU - Shoyama, Yoshinari

AU - Yoshikai, Kazuyoshi

AU - Shoyama, Yukihiro

AU - Morimoto, Satoshi

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Y1 - 2007/6/26

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AB - Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.

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