TY - JOUR
T1 - Carbon and hydrogen isotopic fractionation during lipid biosynthesis in a higher plant (Cryptomeria japonica)
AU - Chikaraishi, Yoshito
AU - Naraoka, Hiroshi
AU - Poulson, Simon R.
N1 - Funding Information:
We thank Mr. Naito for the assistance in the field. This work was supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists (Y.C.) and a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Culture and Technology (H.N.).
PY - 2004/2
Y1 - 2004/2
N2 - Compound-specific carbon and hydrogen isotopic compositions of lipid biomolecules (n-alkanes, n-alkanoic acids, n-alkanols, sesquiterpenes, diterpenes, phytol, diterpenols and β-sitosterol), extracted from Cryptomeria japonica leaves, were determined in order to understand isotopic fractionations occurring during lipid biosynthesis in this species. All lipid biomolecules were depleted in both 13C and D relative to bulk tissue and ambient water, respectively. n-Alkyl lipids associated with the acetogenic pathway were depleted in 13C relative to bulk tissue by 2.4-9.9‰ and depleted in D relative to ambient water by 91-152‰. C15- and C30-isoprenoid lipids (sesquiterpenes, squalene and β-sitosterol) associated with the mevalonic-acid pathway are depleted in 13C relative to bulk tissue by 1.7-3.1‰ and depleted in D relative to ambient water by 212-238‰. C20-isoprenoid lipids (phytol and diterpenoids) associated with the non-mevalonic-acid pathway were depleted in 13C relative to bulk tissue by 4.6-5.9‰ and depleted in D relative to ambient water by 238-303‰. Phytol was significantly depleted in D by amounts up to 65‰ relative to other C 20 isoprenoid lipids. The acetogenic, mevalonic-acid and non-mevalonic-acid pathways were clearly discriminated using a cross-plot between the carbon and hydrogen isotopic fractionations.
AB - Compound-specific carbon and hydrogen isotopic compositions of lipid biomolecules (n-alkanes, n-alkanoic acids, n-alkanols, sesquiterpenes, diterpenes, phytol, diterpenols and β-sitosterol), extracted from Cryptomeria japonica leaves, were determined in order to understand isotopic fractionations occurring during lipid biosynthesis in this species. All lipid biomolecules were depleted in both 13C and D relative to bulk tissue and ambient water, respectively. n-Alkyl lipids associated with the acetogenic pathway were depleted in 13C relative to bulk tissue by 2.4-9.9‰ and depleted in D relative to ambient water by 91-152‰. C15- and C30-isoprenoid lipids (sesquiterpenes, squalene and β-sitosterol) associated with the mevalonic-acid pathway are depleted in 13C relative to bulk tissue by 1.7-3.1‰ and depleted in D relative to ambient water by 212-238‰. C20-isoprenoid lipids (phytol and diterpenoids) associated with the non-mevalonic-acid pathway were depleted in 13C relative to bulk tissue by 4.6-5.9‰ and depleted in D relative to ambient water by 238-303‰. Phytol was significantly depleted in D by amounts up to 65‰ relative to other C 20 isoprenoid lipids. The acetogenic, mevalonic-acid and non-mevalonic-acid pathways were clearly discriminated using a cross-plot between the carbon and hydrogen isotopic fractionations.
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U2 - 10.1016/j.phytochem.2003.12.003
DO - 10.1016/j.phytochem.2003.12.003
M3 - Article
C2 - 14751303
AN - SCOPUS:0742324900
VL - 65
SP - 323
EP - 330
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
IS - 3
ER -