Carbon sources of natural cyanamide in Vicia villosa subsp. varia

Tsunashi Kamo, Ryohei Kasahara, Shun Abe, Mitsuru Hirota, Mami Sugano, Hiroko Yamaya, Syuntaro Hiradate, Yoshiharu Fujii

Research output: Contribution to journalArticlepeer-review


The 13C labels of [13C]carbon dioxide and D-[ 13C6]glucose were incorporated into cyanamide (NH2CN) when they were administered to Vicia villosa subsp. varia shoots. In contrast, the administration of sodium [2,3-13C2]pyruvate did not affect the relative area of the [M + 1]+ ion of cyanamide in the gas chromatography-mass spectrometry analysis. [2,3-13C 2]Pyruvate was incorporated into organic acids that are part of the citric acid cycle, such as succinate and fumarate, confirming that the shoots absorbed and metabolised it. These observations demonstrated that the carbon atom of cyanamide is derived from any of the carbohydrates that are present upstream of pyruvate in the metabolic pathway.

Original languageEnglish
Pages (from-to)1637-1642
Number of pages6
JournalNatural Product Research
Issue number17
Publication statusPublished - Oct 20 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry


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