Abstract
The 13C labels of [13C]carbon dioxide and D-[ 13C6]glucose were incorporated into cyanamide (NH2CN) when they were administered to Vicia villosa subsp. varia shoots. In contrast, the administration of sodium [2,3-13C2]pyruvate did not affect the relative area of the [M + 1]+ ion of cyanamide in the gas chromatography-mass spectrometry analysis. [2,3-13C 2]Pyruvate was incorporated into organic acids that are part of the citric acid cycle, such as succinate and fumarate, confirming that the shoots absorbed and metabolised it. These observations demonstrated that the carbon atom of cyanamide is derived from any of the carbohydrates that are present upstream of pyruvate in the metabolic pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 1637-1642 |
| Number of pages | 6 |
| Journal | Natural Product Research |
| Volume | 24 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - Oct 20 2010 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Plant Science
- Organic Chemistry
Cite this
Carbon sources of natural cyanamide in Vicia villosa subsp. varia. / Kamo, Tsunashi; Kasahara, Ryohei; Abe, Shun; Hirota, Mitsuru; Sugano, Mami; Yamaya, Hiroko; Hiradate, Syuntaro; Fujii, Yoshiharu.
In: Natural Product Research, Vol. 24, No. 17, 20.10.2010, p. 1637-1642.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Carbon sources of natural cyanamide in Vicia villosa subsp. varia
AU - Kamo, Tsunashi
AU - Kasahara, Ryohei
AU - Abe, Shun
AU - Hirota, Mitsuru
AU - Sugano, Mami
AU - Yamaya, Hiroko
AU - Hiradate, Syuntaro
AU - Fujii, Yoshiharu
PY - 2010/10/20
Y1 - 2010/10/20
N2 - The 13C labels of [13C]carbon dioxide and D-[ 13C6]glucose were incorporated into cyanamide (NH2CN) when they were administered to Vicia villosa subsp. varia shoots. In contrast, the administration of sodium [2,3-13C2]pyruvate did not affect the relative area of the [M + 1]+ ion of cyanamide in the gas chromatography-mass spectrometry analysis. [2,3-13C 2]Pyruvate was incorporated into organic acids that are part of the citric acid cycle, such as succinate and fumarate, confirming that the shoots absorbed and metabolised it. These observations demonstrated that the carbon atom of cyanamide is derived from any of the carbohydrates that are present upstream of pyruvate in the metabolic pathway.
AB - The 13C labels of [13C]carbon dioxide and D-[ 13C6]glucose were incorporated into cyanamide (NH2CN) when they were administered to Vicia villosa subsp. varia shoots. In contrast, the administration of sodium [2,3-13C2]pyruvate did not affect the relative area of the [M + 1]+ ion of cyanamide in the gas chromatography-mass spectrometry analysis. [2,3-13C 2]Pyruvate was incorporated into organic acids that are part of the citric acid cycle, such as succinate and fumarate, confirming that the shoots absorbed and metabolised it. These observations demonstrated that the carbon atom of cyanamide is derived from any of the carbohydrates that are present upstream of pyruvate in the metabolic pathway.
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UR - http://www.scopus.com/inward/citedby.url?scp=77958187126&partnerID=8YFLogxK
U2 - 10.1080/14786419.2010.482050
DO - 10.1080/14786419.2010.482050
M3 - Article
VL - 24
SP - 1637
EP - 1642
JO - Natural Product Research
JF - Natural Product Research
SN - 1478-6419
IS - 17
ER -