Carboxylation reactions of pyruvate

Ferredoxin oxidoreductase and 2-oxoglutarate: Ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6

Ki Suk Yoon, M. Ishii, T. Kodama, Y. Igarashi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Enzymatic reactions involving pyruvate:ferredoxin oxidoreductase and 2-oxoglutarate:ferredoxin oxidoreductase from a thermophilic, aerobic, chemolithoautotrophic, and hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, were investigated as the CO2 exchange reaction and CO2 fixation reaction using ferredoxin isolated from the same organism as a reductant. The reduced ferredoxin was required in the pyruvate synthetic reaction and the 2-oxoglutarate synthetic reaction by a cell extract that had been treated with a PD-10 column to remove the low molecular weight substances. [14C]Pyruvate and [14C]2-oxoglutarate were detected as products of pyruvate synthetic and 2-oxoglutarate synthetic reactions, respectively. Further evidence for the operation of pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate:ferredoxin oxidoreductase was obtained from experiments on CO2 exchange reactions using the purified enzymes.

Original languageEnglish
Pages (from-to)510-513
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number3
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

2-oxoglutarate synthase
Hydrogenobacter
pyruvate synthase
Pyruvate Synthase
Carboxylation
ferredoxins
carboxylation
oxidoreductases
Pyruvic Acid
Ferredoxins
hydrogen-oxidizing bacteria
Reducing Agents
Cell Extracts
reducing agents
Hydrogen
Bacteria
enzymatic reactions
Molecular Weight
Molecular weight
Enzymes

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Enzymatic reactions involving pyruvate:ferredoxin oxidoreductase and 2-oxoglutarate:ferredoxin oxidoreductase from a thermophilic, aerobic, chemolithoautotrophic, and hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, were investigated as the CO2 exchange reaction and CO2 fixation reaction using ferredoxin isolated from the same organism as a reductant. The reduced ferredoxin was required in the pyruvate synthetic reaction and the 2-oxoglutarate synthetic reaction by a cell extract that had been treated with a PD-10 column to remove the low molecular weight substances. [14C]Pyruvate and [14C]2-oxoglutarate were detected as products of pyruvate synthetic and 2-oxoglutarate synthetic reactions, respectively. Further evidence for the operation of pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate:ferredoxin oxidoreductase was obtained from experiments on CO2 exchange reactions using the purified enzymes.",
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year = "1997",
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T2 - Ferredoxin oxidoreductase and 2-oxoglutarate: Ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6

AU - Yoon, Ki Suk

AU - Ishii, M.

AU - Kodama, T.

AU - Igarashi, Y.

PY - 1997

Y1 - 1997

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AB - Enzymatic reactions involving pyruvate:ferredoxin oxidoreductase and 2-oxoglutarate:ferredoxin oxidoreductase from a thermophilic, aerobic, chemolithoautotrophic, and hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, were investigated as the CO2 exchange reaction and CO2 fixation reaction using ferredoxin isolated from the same organism as a reductant. The reduced ferredoxin was required in the pyruvate synthetic reaction and the 2-oxoglutarate synthetic reaction by a cell extract that had been treated with a PD-10 column to remove the low molecular weight substances. [14C]Pyruvate and [14C]2-oxoglutarate were detected as products of pyruvate synthetic and 2-oxoglutarate synthetic reactions, respectively. Further evidence for the operation of pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate:ferredoxin oxidoreductase was obtained from experiments on CO2 exchange reactions using the purified enzymes.

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