Carboxylation Reactions of Pyruvate: Ferredoxin Oxidoreductase and 2-Oxoglutarate: Ferredoxin Oxidoreductase from Hydrogenobacter thermophilus TK-6

Ki Seok Yoon, Masaharu Ishii, Tohru Kodama, Yasuo Igarashi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Enzymatic reactions involving pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate: ferredoxin oxidoreductase from a thermophilic, aerobic, chemolithoautotrophic, and hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, were investigated as the CO2 exchange reaction and CO2 fixation reaction using ferredoxin isolated from the same organism as a reductant. The reduced ferredoxin was required in the pyruvate synthetic reaction and the 2-oxoglutarate synthetic reaction by a cell extract that had been treated with a PD-10 column to remove the low molecular weight substances. [14C]Pyruvate and [14C]2-oxoglutarate were detected as products of pyruvate synthetic and 2-oxoglutarate synthetic reactions, respectively. Further evidence for the operation of pyruvate: ferredoxin oxidoreductase and 2-oxoglutarate: ferredoxin oxidoreductase was obtained from experiments on CO2 exchange reactions using the purified enzymes.

Original languageEnglish
Pages (from-to)510-513
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number3
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

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2-oxoglutarate synthase
Pyruvate Synthase
Carboxylation
Pyruvic Acid
Ferredoxins
Reducing Agents
Cell Extracts
Hydrogen
Bacteria
Molecular Weight
Molecular weight
Enzymes
alpha-ketoglutaric acid
Experiments

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Carboxylation Reactions of Pyruvate : Ferredoxin Oxidoreductase and 2-Oxoglutarate: Ferredoxin Oxidoreductase from Hydrogenobacter thermophilus TK-6. / Yoon, Ki Seok; Ishii, Masaharu; Kodama, Tohru; Igarashi, Yasuo.

In: Bioscience, Biotechnology and Biochemistry, Vol. 61, No. 3, 01.01.1997, p. 510-513.

Research output: Contribution to journalArticle

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