Carnosine induces intestinal cells to secrete exosomes that activate neuronal cells

Yuka Sugihara, Shiori Onoue, Kosuke Tashiro, Mikako Sato, Takanori Hasegawa, Yoshinori Katakura

Research output: Contribution to journalArticle

Abstract

Recently, we showed that imidazole dipeptide such as carnosine contained abundantly in chicken breast meat improves brain function in a double-blind randomized controlled trial. However, the underlying molecular mechanisms remain unknown. Here, we investigated whether carnosine activates intestinal epithelial cells and induces the secretion of factors that activate brain function. We focused on exosomes derived from intestinal epithelial cells as mediators of brain-gut interaction. Results showed that exosomes derived from Caco-2 cells treated with carnosine significantly induced neurite growth in SH-SY5Y cells. To clarify the molecular basis of this finding, we performed integrated analysis of microRNAs (miRNAs) with altered expression in exosomes in response to carnosine treatment and mRNAs with altered expression in target cells in response to exosome treatment to identify related miRNAs and their target genes. The combination of miR-6769-5p and its target gene ATXN1 was found to be involved in the exosome-induced activation of neuronal cells.

Original languageEnglish
Article numbere0217394
JournalPloS one
Volume14
Issue number5
DOIs
Publication statusPublished - May 1 2019

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exosomes
Carnosine
Exosomes
carnosine
neurons
Brain
MicroRNAs
microRNA
brain
Genes
cells
epithelial cells
Epithelial Cells
Meats
Dipeptides
imidazoles
neurites
Caco-2 Cells
dipeptides
breast meat

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Carnosine induces intestinal cells to secrete exosomes that activate neuronal cells. / Sugihara, Yuka; Onoue, Shiori; Tashiro, Kosuke; Sato, Mikako; Hasegawa, Takanori; Katakura, Yoshinori.

In: PloS one, Vol. 14, No. 5, e0217394, 01.05.2019.

Research output: Contribution to journalArticle

Sugihara, Yuka ; Onoue, Shiori ; Tashiro, Kosuke ; Sato, Mikako ; Hasegawa, Takanori ; Katakura, Yoshinori. / Carnosine induces intestinal cells to secrete exosomes that activate neuronal cells. In: PloS one. 2019 ; Vol. 14, No. 5.
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