Abstract
To examine the possibility that staurosporine is applicable for the treatment of abnormal scar formation such as hypertrophic scar and keloid, the cellular process during staurosporine-induced apoptosis was analyzed in myofibroblasts isolated from a rat granulation tissue pouch. Staurosporine induced myofibroblast apoptosis in a time- and a dose-dependent manner with typical morphological changes. Staurosporine activated caspase-3 up to 3.6-fold by cleaving pro-caspase-3 to active forms. Alpha-smooth muscle actin was degraded during staurosporine-induced apoptosis. The degradation of α-smooth muscle actin was detected at an early stage of staurosporine-induced apoptosis. Recombinant active caspase-3 and staurosporine-stimulated caspase-3 both cleaved purified α-smooth muscle actin in vitro. These results suggested that α-smooth muscle actin is directly degraded by caspase-3 in response to apoptotic stimuli in myofibroblasts.
Original language | English |
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Pages (from-to) | 94P-95P |
Journal | Folia Pharmacologica Japonica |
Volume | 120 |
Issue number | SUPPL. 1 |
Publication status | Published - 2002 |
All Science Journal Classification (ASJC) codes
- Pharmacology