Ca2+ Binding to Cardiac Troponin C in the Myofilament Lattice and Its Relation to the Myofibrillar ATPase Activity

Sachio Morimoto, Iwao Ohtsuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The Ca2+‐binding properties of troponin C in the intact myofilament lattice and their relation to the activation of ATPase were investigated with isolated porcine cardiac myofibrils. Ca2+ binding, which is composed of two classes of binding sites with different affinities (classes 1 and 2), was clearly detected by a novel method for subtracting the large background activity of myofibrillar Ca2+ binding. The classes 1 and 2 were equivalent stoichiometrically to the two high‐affinity sites (sites III and IV) and a single low‐affinity site (site II) of troponin C. In the presence of ATP, positive cooperativity was observed in the Ca2+ binding of class‐2 sites and the Hill equation parameters were in excellent agreement with those for the Ca2+‐activated myofibrillar ATPase activity, which indicated that the activation of ATPase is a linear function of the Ca2+ occupancy of site II. In the absence of ATP, a marked increase in the affinity of only class‐2 sites was observed while the cooperativity was lost. These results provide direct evidence that some feedback mechanism exists between myosin crossbridge attachment and the Ca2+ binding to site II of troponin C, which may thus confer positive cooperativity on the Ca2+ activation of myofibrillar ATPase activity.

Original languageEnglish
Pages (from-to)597-602
Number of pages6
JournalEuropean Journal of Biochemistry
Volume226
Issue number2
DOIs
Publication statusPublished - Jan 1 1994

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Troponin C
Myofibrils
Adenosine Triphosphatases
Chemical activation
Binding Sites
Adenosine Triphosphate
Myosins
Swine
Feedback

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Ca2+ Binding to Cardiac Troponin C in the Myofilament Lattice and Its Relation to the Myofibrillar ATPase Activity. / Morimoto, Sachio; Ohtsuki, Iwao.

In: European Journal of Biochemistry, Vol. 226, No. 2, 01.01.1994, p. 597-602.

Research output: Contribution to journalArticle

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