Ca2+ influx evoked by inositol-3,4,5,6-tetrakisphosphate in ras-transformed NIH/3T3 fibroblasts

Minako Hashii, Masato Hirata, Shoichiro Ozaki, Yoshinori Nozawa, Haruhiro Higashida

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Infusion of inositol-3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4) from the patch pipette into the cytoplasm, produced a biphasic intracellular free Ca2+ concentration ([Ca2+]i) increase in ras-transformed NIH/3T3 (DT) cells. The Ins(3,4,5,6)P4-induced increase in DT cells depended upon extracellular Ca2+ and was enhanced by membrane hyperpolarization. Identical [Ca2+]i increases were observed with intracellular application of inositol-1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4) and inositol-1,3,4,6-tetrakisphosphate but not with inositol-1,2,4,5-tetrakisphosphate, inositol-1,4,5-trisphosphate or inositol-1,3,4,5,6-pentakisphosphate. Stimulation of DT cells with bradykinin increased the levels of Ins(3,4,5,6)P4 and Ins(1,3,4,5)P4. These results suggest that Ins(3,4,5,6)P4 may serve as a second messenger for continuous Ca2+ influx along with other tetrakisphosphates downstream from bradykinin receptors in DT cells.

Original languageEnglish
Pages (from-to)276-280
Number of pages5
JournalFEBS Letters
Volume340
Issue number3
DOIs
Publication statusPublished - Mar 7 1994

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Fibroblasts
Bradykinin Receptors
NIH 3T3 Cells
Inositol 1,4,5-Trisphosphate
Second Messenger Systems
Bradykinin
Cytoplasm
inositol-3,4,5,6-tetrakisphosphate
Membranes
inositol-1,3,4,5-tetrakisphosphate

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Ca2+ influx evoked by inositol-3,4,5,6-tetrakisphosphate in ras-transformed NIH/3T3 fibroblasts. / Hashii, Minako; Hirata, Masato; Ozaki, Shoichiro; Nozawa, Yoshinori; Higashida, Haruhiro.

In: FEBS Letters, Vol. 340, No. 3, 07.03.1994, p. 276-280.

Research output: Contribution to journalArticle

Hashii, Minako ; Hirata, Masato ; Ozaki, Shoichiro ; Nozawa, Yoshinori ; Higashida, Haruhiro. / Ca2+ influx evoked by inositol-3,4,5,6-tetrakisphosphate in ras-transformed NIH/3T3 fibroblasts. In: FEBS Letters. 1994 ; Vol. 340, No. 3. pp. 276-280.
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