TY - JOUR
T1 - Ca2+-influx gated by inositol-3,4,5,6-tetrakisphosphate in NIH/3T3 fibroblasts
AU - Hashii, Minako
AU - Hirata, Masato
AU - Ozaki, Shoichiro
AU - Nozawa, Yoshinori
AU - Higashida, Haruhiro
PY - 1994/5/15
Y1 - 1994/5/15
N2 - The functional role of three inositol tetrakisphosphate isomers on Ca2+ influx was examined by intracellular application in NIH/3T3 mouse fibroblasts. Infusion of inositol-1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), inositol-1,3,4,6-tetrakisphosphate, and inositol-3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4) from the patch pipette into the cytoplasm produced a transient increase in cytoplasmic free Ca2+ concentration by fura-2 recording in extracellular Ca2+-dependent fashion. Stimulation of NIH/3T3 cells with bradykinin increased the levels of Ins(1,3,4,5)P4 and Ins(3,4,5,6)P4. These results suggest that mouse fibroblast cells possess a Ca2+ influx pathway gated with naturally occuring inositol tetrakisphosphates in response to stimulation with bradykinin.
AB - The functional role of three inositol tetrakisphosphate isomers on Ca2+ influx was examined by intracellular application in NIH/3T3 mouse fibroblasts. Infusion of inositol-1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), inositol-1,3,4,6-tetrakisphosphate, and inositol-3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4) from the patch pipette into the cytoplasm produced a transient increase in cytoplasmic free Ca2+ concentration by fura-2 recording in extracellular Ca2+-dependent fashion. Stimulation of NIH/3T3 cells with bradykinin increased the levels of Ins(1,3,4,5)P4 and Ins(3,4,5,6)P4. These results suggest that mouse fibroblast cells possess a Ca2+ influx pathway gated with naturally occuring inositol tetrakisphosphates in response to stimulation with bradykinin.
UR - http://www.scopus.com/inward/record.url?scp=0028181385&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028181385&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1994.1592
DO - 10.1006/bbrc.1994.1592
M3 - Article
C2 - 8185579
AN - SCOPUS:0028181385
SN - 0006-291X
VL - 200
SP - 1300
EP - 1306
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -