リゾチームの触媒機構:無脊椎動物型とニワトリ型の比較から

Translated title of the contribution: Catalytic Mechanism of Lysozyme Based on the Structures of Invertebrate-type Lysozyme and Chicken-type Lysozyme

阿部 義人

Research output: Contribution to journalArticle

Abstract

<p>Based on the structure of invertebrate-type lyzozyme, <i>Venerupis philippinarum</i> lysozyme (<i>Vp</i>L), we designed Asn46Asp/Asp52Ser or Asn46Glu/Asp52Ser hen egg white lysozyme (HEWL) mutants. Both lysozyme mutants possessed lysozyme acitivity like <i>Vp</i>L and HEWL. Although Asn46Glu/Asp52Ser mutant formed a glycosyl adduct in the reaction with the N-acetylglucosamine oligomer, however, the turnover rate of estimated from the glycosyl formation and decomposition rates was only 20% of the observed hydrolysis rate of the substrate. From these results, we discussed the catalytic mechanism of lysozymes.</p>
Translated title of the contributionCatalytic Mechanism of Lysozyme Based on the Structures of Invertebrate-type Lysozyme and Chicken-type Lysozyme
Original languageJapanese
Pages (from-to)140-143
Number of pages4
Journal生物物理
Volume57
Issue number3
DOIs
Publication statusPublished - 2017

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