Catechol sensor based on ascorbate oxidase immobilized polymer-modified graphite electrode

Toshihiko Imato, Hironori Morioka, William R. Heineman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A selectivity and sensitivity enhanced catechol sensor based on a polymer-modified graphite electrode was fabricated by immobilizing ascorbate oxidase (AO) into a poly(N-vinyl pyrrolidone) (PNVP) membrane. AO was immobilized between two PNVP membrane layers as a sandwiched structure by cross-linking PNVP with gamma radiation. The sensor responses to catechol and ascorbic acid were evaluated by cyclic voltammetry. The sensor showed a selective response to catechol even in the presence of ascorbic acid, which was due to blocking ascorbic acid access to the electrode surface by the enzymatic activity of AO immobilized in the polymer membrane. Compared to a bare electrode without modification with the PNVP membrane, an enhanced response of the sensor to catechol was observed, which was due to preconcentration of catechol into the PNVP membrane. The enzymatic activity of AO immobilized on the sensor surface did not change for at least four days.

Original languageEnglish
Pages (from-to)68-72
Number of pages5
JournalSensors and Actuators: B. Chemical
Volume13
Issue number1-3
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

Ascorbate Oxidase
Graphite electrodes
oxidase
Polymers
graphite
ascorbic acid
membranes
Ascorbic acid
Membranes
electrodes
sensors
Sensors
Ascorbic Acid
polymers
Electrodes
Gamma rays
Cyclic voltammetry
selectivity
N-vinyl-2-pyrrolidinone
catechol

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

Cite this

Catechol sensor based on ascorbate oxidase immobilized polymer-modified graphite electrode. / Imato, Toshihiko; Morioka, Hironori; Heineman, William R.

In: Sensors and Actuators: B. Chemical, Vol. 13, No. 1-3, 01.01.1993, p. 68-72.

Research output: Contribution to journalArticle

Imato, Toshihiko ; Morioka, Hironori ; Heineman, William R. / Catechol sensor based on ascorbate oxidase immobilized polymer-modified graphite electrode. In: Sensors and Actuators: B. Chemical. 1993 ; Vol. 13, No. 1-3. pp. 68-72.
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