Caterpillar color patterns are determined by a two-phase melanin gene prepatterning process

New evidence from tan and laccase2

Ryo Futahashi, Yutaka Banno, Haruhiko Fujiwara

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The larval color patterns in Lepidoptera exhibit splendid diversity, and identifying the genes responsible for pigment distribution is essential to understanding color-pattern evolution. The swallowtail butterfly, Papilio xuthus, is a good candidate for analyzing marking-associated genes because its body markings change dramatically at the final molt. Moreover, the silkworm Bombyx mori is most suitable for identification of lab-generated color mutants because genome information and many color mutants are available. Here, we analyzed the expression pattern of 10 melanin-related genes in P. xuthus, and analyzed whether these genes were responsible for Bombyx larval color mutants. We found that seven genes correlated strongly with the stage-specific larval cuticular markings of P. xuthus, suggesting that, compared with Drosophila, more genes showed marking specificity in lepidopteran larvae. We newly found that the expression of both tan and laccase2 is strongly correlated with the larval black markings in both P. xuthus and B. mori. The results of F2 linkage analysis and mutant analysis strongly suggest that tan is the responsible gene for Bombyx larval color mutant rouge, and that tan is important in emphasizing black markings of lepidopteran larvae. Detailed comparison of temporal and spatial expression patterns showed that larval cuticular markings were regulated at two different phases. Markingspecific expression of oxidizing enzymes preceded the marking-specific expression of melanin synthesis enzymes at mRNA level, which is the reverse of the melanin synthesis step.

Original languageEnglish
Pages (from-to)157-167
Number of pages11
JournalEvolution and Development
Volume12
Issue number2
DOIs
Publication statusPublished - Mar 2010

Fingerprint

melanin
Melanins
caterpillar
Papilio xuthus
insect larvae
Bombyx
Color
color
gene
Genes
mutants
genes
Lepidoptera
Bombyx mori
Larva
enzyme
larva
Papilionidae
Butterflies
synthesis

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Developmental Biology

Cite this

Caterpillar color patterns are determined by a two-phase melanin gene prepatterning process : New evidence from tan and laccase2. / Futahashi, Ryo; Banno, Yutaka; Fujiwara, Haruhiko.

In: Evolution and Development, Vol. 12, No. 2, 03.2010, p. 157-167.

Research output: Contribution to journalArticle

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