Cathepsin B in the Growth of Colorectal Cancer: Suppressive Effect of Leupeptin on the Growth of DMH-induced Rat Colon Neoplasm

Yasumasa Satoh, Toshihiro Higashi, Kazuhiro Nouso, Tetsuya Shiota, Nobuyuki Kinugasa, Keigo Yoshida, Shuji Uematsu, Harushige Nakatsukasa, Yukio Nishimura, Takao Tsuji

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cathepsin B, a thiol protease, has been reported to be involved in cancer progression and metastasis. The suppressive effects of two kinds of protease inhibitors, leupeptin and dietary camostate (FOY-305), on tumorigenesis and progression in 1, 2-dimethylhydrazine (DMH)-induced rat colon neoplasm were examined in relation to tissue cathepsin B activity. Male Donryu rats were treated with leupeptin or FOY-305 during or after the administration of DMH. There were no significant differences in average tumor numbers among all DMH-treated groups. However, the percentage of small tumors was significantly higher in the group in which leupeptin was supplied during DMH administration. This trend was not recognized in the FOY-305-treated groups. The ratio of cathepsin B activity in the tumors to that in the tumor-bearing tissue (T/Tb) was significantly increased with increasing tumor size (P=0.009). The cathepsin B activity levels in the tumor-bearing mucosa in the groups which received leupeptin or FOY-305 following DMH treatment were both significantly lower than that in the group which received neither protease inhibitor (P=0.046 and P= 0.0067, respectively). The results obtained indicate that leupeptin may have suppressed tumor growth by lowering the tissue cathepsin B activity.

Original languageEnglish
Pages (from-to)299-303
Number of pages5
JournalActa medica Okayama
Volume50
Issue number6
Publication statusPublished - Dec 1 1996

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1,2-Dimethylhydrazine
Cathepsin B
Colonic Neoplasms
Rats
Tumors
Colorectal Neoplasms
Growth
Bearings (structural)
Neoplasms
Tissue
Protease Inhibitors
leupeptin
Sulfhydryl Compounds
Peptide Hydrolases
camostat
Carcinogenesis
Mucous Membrane
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Satoh, Y., Higashi, T., Nouso, K., Shiota, T., Kinugasa, N., Yoshida, K., ... Tsuji, T. (1996). Cathepsin B in the Growth of Colorectal Cancer: Suppressive Effect of Leupeptin on the Growth of DMH-induced Rat Colon Neoplasm. Acta medica Okayama, 50(6), 299-303.

Cathepsin B in the Growth of Colorectal Cancer : Suppressive Effect of Leupeptin on the Growth of DMH-induced Rat Colon Neoplasm. / Satoh, Yasumasa; Higashi, Toshihiro; Nouso, Kazuhiro; Shiota, Tetsuya; Kinugasa, Nobuyuki; Yoshida, Keigo; Uematsu, Shuji; Nakatsukasa, Harushige; Nishimura, Yukio; Tsuji, Takao.

In: Acta medica Okayama, Vol. 50, No. 6, 01.12.1996, p. 299-303.

Research output: Contribution to journalArticle

Satoh, Y, Higashi, T, Nouso, K, Shiota, T, Kinugasa, N, Yoshida, K, Uematsu, S, Nakatsukasa, H, Nishimura, Y & Tsuji, T 1996, 'Cathepsin B in the Growth of Colorectal Cancer: Suppressive Effect of Leupeptin on the Growth of DMH-induced Rat Colon Neoplasm', Acta medica Okayama, vol. 50, no. 6, pp. 299-303.
Satoh, Yasumasa ; Higashi, Toshihiro ; Nouso, Kazuhiro ; Shiota, Tetsuya ; Kinugasa, Nobuyuki ; Yoshida, Keigo ; Uematsu, Shuji ; Nakatsukasa, Harushige ; Nishimura, Yukio ; Tsuji, Takao. / Cathepsin B in the Growth of Colorectal Cancer : Suppressive Effect of Leupeptin on the Growth of DMH-induced Rat Colon Neoplasm. In: Acta medica Okayama. 1996 ; Vol. 50, No. 6. pp. 299-303.
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