Cathepsin D deficiency induces oxidative damage in brain pericytes and impairs the blood-brain barrier

Ryo Okada, Hiro Take, Aiqin Zhu, Junjun Ni, Jingqi Zhang, Yoshimine Yoshito, Christoph Peters, Paul Saftig, Hiroshi Nakanishi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Recent evidence suggests that peripheral blood mononuclear cells (PBMCs) contribute to the pathogenesis of neuropathological changes in patients with neuronal ceroid lipofuscinosis (NCL) and lysosomal storage diseases. In order to examine the possible increase in the permeability of the blood-brain-barrier (BBB) and resultant infiltration of PBMCs due to cathepsin D (CatD) deficiency, a process underlying the onset of congenital NCL, we examined structural changes in brain vessels in CatD-/- mice. Consequently, the mean diameter of the brain vessels in the cerebral cortex on postnatal day 24 (P24) was significantly larger in CatD-/- mice than in wild-type mice. Furthermore, the mean number of brain pericytes in CatD-/- mice began to decline significantly on P16 and almost disappeared on P24, and oxidative DNA damage was first detected in brain pericytes on P12. Examinations with electron microscopy revealed that brain pericytes were laden with dense granular bodies, cytoplasmic vacuoles and lipid droplets. The infiltration of PBMCs characterized by segmented nucleus laden with dense granular bodies was also noted in the cerebral cortex of CatD-/- mice. When primary cultured microglia prepared from enhanced green fluorescent protein (GFP)-expressing transgenic rats were injected into the common carotid artery, GFP-positive microglia were detected in the brain parenchyma of CatD-/-, but not wild-type, mice. Moreover, pepstatin A, a specific aspartic protease inhibitor, induced mitochondria-derived reactive oxygen species (ROS) production in the isolated brain pericytes, which decreased the cell viability. These observations suggest that increased lysosomal storage due to CatD deficiency causes oxidative damage in brain pericytes, subsequently resulting in an increased vessel diameter, enhanced permeability of the BBB and the infiltration of PBMCs. Therefore, protecting brain pericytes against lysosomal storage-induced oxidative stress may represent an alternative treatment strategy for congenital NCL.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume64
DOIs
Publication statusPublished - Jan 1 2015

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Pericytes
Cathepsin D
Blood-Brain Barrier
Brain
Blood Cells
Neuronal Ceroid-Lipofuscinoses
Microglia
Cerebral Cortex
Permeability
Lysosomal Storage Diseases
Transgenic Rats
Common Carotid Artery
Vacuoles
Green Fluorescent Proteins
Protease Inhibitors
DNA Damage
Reactive Oxygen Species
Cell Survival
Electron Microscopy
Mitochondria

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Cathepsin D deficiency induces oxidative damage in brain pericytes and impairs the blood-brain barrier. / Okada, Ryo; Take, Hiro; Zhu, Aiqin; Ni, Junjun; Zhang, Jingqi; Yoshito, Yoshimine; Peters, Christoph; Saftig, Paul; Nakanishi, Hiroshi.

In: Molecular and Cellular Neuroscience, Vol. 64, 01.01.2015, p. 51-60.

Research output: Contribution to journalArticle

Okada, Ryo ; Take, Hiro ; Zhu, Aiqin ; Ni, Junjun ; Zhang, Jingqi ; Yoshito, Yoshimine ; Peters, Christoph ; Saftig, Paul ; Nakanishi, Hiroshi. / Cathepsin D deficiency induces oxidative damage in brain pericytes and impairs the blood-brain barrier. In: Molecular and Cellular Neuroscience. 2015 ; Vol. 64. pp. 51-60.
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