Cationic comb-type copolymers do not cause collapse but shrinkage of DNA molecules

Naotaka Sonda, Masanori Hirano, Naohiko Shimada, Arihiro Kano, Satoru Kidoaki, Atsushi Maruyama

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


A flow-stretching assay was employed to elucidate polycation/DNA interactions at the single-molecule level. Bacteriophage λ-DNA (48.5 kbp) was attached at one end to a PEGmodified glass surface and stretched by buffer flow. The stretched DNA had an approximate length of 12 μm. Upon injection of polylysine homopolymer, the DNA folded into a globule structure within a few seconds. Injection of a polylysine graft copolymer having hydrophilic dextran side chains also induced collapse of the stretched DNA. In comparison to these polymers, a copolymer with higher graft content did not cause DNA collapse but rather caused 25% shrinkage of the extended DNA. These results were compared with those observed with unstretched DNA.

Original languageEnglish
Pages (from-to)250-251
Number of pages2
JournalChemistry Letters
Issue number3
Publication statusPublished - 2011

All Science Journal Classification (ASJC) codes

  • Chemistry(all)


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