Caveolae-independent activation of protein kinase A in rat neonatal myocytes

Masashi Fukutomi, Motohiro Nishida, Yoshiko Maruyama, Hiroyuki Kobayashi, Hitoshi Kurose

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cardiomyocytes express both β1- and β2- adrenergic receptors, and these receptors play a differential role in chronotropic and inotropic effects of the heart. Caveolae are known as an important regulator of G-protein-coupled receptor signaling. In the present report, we examined whether caveolae have a role in β-adrenergic receptor-stimulated cAMP production and protein kinase A activation in neonatal myocytes. Isoproterenol-stimulated cAMP production was mediated by β1- and β2-subtypes, which depends on the receptor number of each subtype. However, protein kinase A activation was exclusively mediated by the β1-subtype. Disruption of caveolae by methyl-β-cyclodextrin treatment did not affect the relative contribution of subtypes to isoproterenol-stimulated cAMP production, β1- Subtype-mediated protein kinase A activation was also not affected by the disruption of caveolae. These results suggest that β1-adrenergic receptor-mediated protein kinase A activation is compartmentalized and independent of caveolae.

Original languageEnglish
Pages (from-to)168-174
Number of pages7
JournalJournal of Pharmacological Sciences
Volume98
Issue number2
DOIs
Publication statusPublished - Jun 1 2005

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

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