Cbx2, a polycomb group gene, is required for Sry gene expression in mice

Yuko Katoh-Fukui, Kanako Miyabayashi, Tomoko Komatsu, Akiko Owaki, Takashi Baba, Yuichi Shima, Tomohide Kidokoro, Yoshiakira Kanai, Andreas Schedl, Dagmar Wilhelm, Peter Koopman, Yasushi Okuno, Ken Ichirou Morohashi

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Mice lacking the function of the polycomb group protein CBX2 (chromobox homolog 2; also known as M33) show defects in gonadal, adrenal, and splenic development. In particular, XY knockout (KO) mice develop ovaries but not testes, and the gonads are hypoplastic in both sexes. However, how CBX2 regulates development of these tissues remains largely unknown. In the present study, weused microarray, RT-PCR, and immunohistochemical analyses to show that the expression of Sry, Sox9, Lhx9, Ad4BP/SF-1, Dax-1, Gata4, Arx, and Dmrt1, genes encoding transcription factors essential for gonadal development, is affected in Cbx2 KO gonads. Male-to-female sex reversal in Cbx2 KO mice was rescued by crossing them with transgenic mice displaying forced expression of Sry or Sox9. However, testes remained hypoplastic in these mice, indicating that the size and the sex of the gonad are determined by different sets of genes. Our study implicates Cbx2 in testis differentiation through regulating Sry gene expression.

Original languageEnglish
Pages (from-to)913-924
Number of pages12
JournalEndocrinology
Volume153
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

Fingerprint

sry Genes
Gonads
Testis
Gene Expression
Knockout Mice
Polycomb-Group Proteins
Genes
Transgenic Mice
Ovary
Transcription Factors
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Cbx2, a polycomb group gene, is required for Sry gene expression in mice. / Katoh-Fukui, Yuko; Miyabayashi, Kanako; Komatsu, Tomoko; Owaki, Akiko; Baba, Takashi; Shima, Yuichi; Kidokoro, Tomohide; Kanai, Yoshiakira; Schedl, Andreas; Wilhelm, Dagmar; Koopman, Peter; Okuno, Yasushi; Morohashi, Ken Ichirou.

In: Endocrinology, Vol. 153, No. 2, 01.02.2012, p. 913-924.

Research output: Contribution to journalArticle

Katoh-Fukui, Y, Miyabayashi, K, Komatsu, T, Owaki, A, Baba, T, Shima, Y, Kidokoro, T, Kanai, Y, Schedl, A, Wilhelm, D, Koopman, P, Okuno, Y & Morohashi, KI 2012, 'Cbx2, a polycomb group gene, is required for Sry gene expression in mice', Endocrinology, vol. 153, no. 2, pp. 913-924. https://doi.org/10.1210/en.2011-1055
Katoh-Fukui Y, Miyabayashi K, Komatsu T, Owaki A, Baba T, Shima Y et al. Cbx2, a polycomb group gene, is required for Sry gene expression in mice. Endocrinology. 2012 Feb 1;153(2):913-924. https://doi.org/10.1210/en.2011-1055
Katoh-Fukui, Yuko ; Miyabayashi, Kanako ; Komatsu, Tomoko ; Owaki, Akiko ; Baba, Takashi ; Shima, Yuichi ; Kidokoro, Tomohide ; Kanai, Yoshiakira ; Schedl, Andreas ; Wilhelm, Dagmar ; Koopman, Peter ; Okuno, Yasushi ; Morohashi, Ken Ichirou. / Cbx2, a polycomb group gene, is required for Sry gene expression in mice. In: Endocrinology. 2012 ; Vol. 153, No. 2. pp. 913-924.
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