cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cells

Yasuyuki Hayashi, Tohru Kiyono, Masatoshi Fujita, Masahide Ishibashi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A novel cDNA fragment, named cca1 (confluent 3Y1 cell-associated 1), was previously isolated on the basis of preferential accumulation of the corresponding mRNA in growth-arrested confluent but not in growing subconfluent rat 3Y1 cells. The cca1 cDNA was found to consist of 5022 nucleotides with an open reading frame of 1905 nucleotides, encoding a protein of 635 amino acids. Unlike the 3Y1 cell case, cca1 mRNA was not detected in confluent 3Y1 BU, 3Y1 BU/pTK, 3Y1-16E6, or F2408 cells, whose growth patterns monitored by phalloidin staining and bromodeoxyuridine incorporation were different from those of the confluent 3Y1 cells. A restoration of the confluent 3Y1-type growth pattern was observed in the cca1 cDNA-introduced 3Y1 BU and 3Y1 BU/pTK cells after reaching confluence but not in the cDNA-introduced 3Y1-16E6 or F2408 cells. The results allow us to conclude that cca1 is required but not sufficient for formation of growth- arrested confluent monolayer of 3Y1 cells.

Original languageEnglish
Pages (from-to)18082-18086
Number of pages5
JournalJournal of Biological Chemistry
Volume272
Issue number29
DOIs
Publication statusPublished - Jul 18 1997
Externally publishedYes

Fingerprint

Rats
Monolayers
Complementary DNA
Growth
Nucleotides
Phalloidine
Messenger RNA
Cell growth
Bromodeoxyuridine
Restoration
Amino Acids
Proteins
Open Reading Frames
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cells. / Hayashi, Yasuyuki; Kiyono, Tohru; Fujita, Masatoshi; Ishibashi, Masahide.

In: Journal of Biological Chemistry, Vol. 272, No. 29, 18.07.1997, p. 18082-18086.

Research output: Contribution to journalArticle

Hayashi, Yasuyuki ; Kiyono, Tohru ; Fujita, Masatoshi ; Ishibashi, Masahide. / cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cells. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 29. pp. 18082-18086.
@article{1f756108a6c340d9aa00b062f01c54af,
title = "cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cells",
abstract = "A novel cDNA fragment, named cca1 (confluent 3Y1 cell-associated 1), was previously isolated on the basis of preferential accumulation of the corresponding mRNA in growth-arrested confluent but not in growing subconfluent rat 3Y1 cells. The cca1 cDNA was found to consist of 5022 nucleotides with an open reading frame of 1905 nucleotides, encoding a protein of 635 amino acids. Unlike the 3Y1 cell case, cca1 mRNA was not detected in confluent 3Y1 BU, 3Y1 BU/pTK, 3Y1-16E6, or F2408 cells, whose growth patterns monitored by phalloidin staining and bromodeoxyuridine incorporation were different from those of the confluent 3Y1 cells. A restoration of the confluent 3Y1-type growth pattern was observed in the cca1 cDNA-introduced 3Y1 BU and 3Y1 BU/pTK cells after reaching confluence but not in the cDNA-introduced 3Y1-16E6 or F2408 cells. The results allow us to conclude that cca1 is required but not sufficient for formation of growth- arrested confluent monolayer of 3Y1 cells.",
author = "Yasuyuki Hayashi and Tohru Kiyono and Masatoshi Fujita and Masahide Ishibashi",
year = "1997",
month = "7",
day = "18",
doi = "10.1074/jbc.272.29.18082",
language = "English",
volume = "272",
pages = "18082--18086",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "29",

}

TY - JOUR

T1 - cca1 is required for formation of growth-arrested confluent monolayer of rat 3Y1 cells

AU - Hayashi, Yasuyuki

AU - Kiyono, Tohru

AU - Fujita, Masatoshi

AU - Ishibashi, Masahide

PY - 1997/7/18

Y1 - 1997/7/18

N2 - A novel cDNA fragment, named cca1 (confluent 3Y1 cell-associated 1), was previously isolated on the basis of preferential accumulation of the corresponding mRNA in growth-arrested confluent but not in growing subconfluent rat 3Y1 cells. The cca1 cDNA was found to consist of 5022 nucleotides with an open reading frame of 1905 nucleotides, encoding a protein of 635 amino acids. Unlike the 3Y1 cell case, cca1 mRNA was not detected in confluent 3Y1 BU, 3Y1 BU/pTK, 3Y1-16E6, or F2408 cells, whose growth patterns monitored by phalloidin staining and bromodeoxyuridine incorporation were different from those of the confluent 3Y1 cells. A restoration of the confluent 3Y1-type growth pattern was observed in the cca1 cDNA-introduced 3Y1 BU and 3Y1 BU/pTK cells after reaching confluence but not in the cDNA-introduced 3Y1-16E6 or F2408 cells. The results allow us to conclude that cca1 is required but not sufficient for formation of growth- arrested confluent monolayer of 3Y1 cells.

AB - A novel cDNA fragment, named cca1 (confluent 3Y1 cell-associated 1), was previously isolated on the basis of preferential accumulation of the corresponding mRNA in growth-arrested confluent but not in growing subconfluent rat 3Y1 cells. The cca1 cDNA was found to consist of 5022 nucleotides with an open reading frame of 1905 nucleotides, encoding a protein of 635 amino acids. Unlike the 3Y1 cell case, cca1 mRNA was not detected in confluent 3Y1 BU, 3Y1 BU/pTK, 3Y1-16E6, or F2408 cells, whose growth patterns monitored by phalloidin staining and bromodeoxyuridine incorporation were different from those of the confluent 3Y1 cells. A restoration of the confluent 3Y1-type growth pattern was observed in the cca1 cDNA-introduced 3Y1 BU and 3Y1 BU/pTK cells after reaching confluence but not in the cDNA-introduced 3Y1-16E6 or F2408 cells. The results allow us to conclude that cca1 is required but not sufficient for formation of growth- arrested confluent monolayer of 3Y1 cells.

UR - http://www.scopus.com/inward/record.url?scp=0030792143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030792143&partnerID=8YFLogxK

U2 - 10.1074/jbc.272.29.18082

DO - 10.1074/jbc.272.29.18082

M3 - Article

VL - 272

SP - 18082

EP - 18086

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 29

ER -