CCAAT/enhancer-binding protein β expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis

Satoshi Yoshioka, Yasuo Miura, Hisayuki Yao, Sakiko Satake, Yoshihiro Hayashi, Akihiro Tamura, Terutoshi Hishita, Tatsuo Ichinohe, Hideyo Hirai, Akifumi Takaor-Kondo, Taira Maekawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) regulates the differentiation of a variety of cell types. Here, the role of C/EBPβ expressed by bone marrow mesenchymal stromal cells (BMMSCs) in B-cell lymphopoiesis was examined. The size of the precursor B-cell population in bone marrow was reduced in C/EBPβ-knockout (KO) mice. When bone marrow cells from C/EBPβ-KO mice were transplanted into lethally irradiated wild-type (WT) mice, which provide a normal bone marrow microenvironment, the size of the precursor B-cell population was restored to a level equivalent to that generated by WT bone marrow cells. In coculture experiments, BMMSCs from C/EBPβ-KO mice did not support the differentiation of WT c-Kit+ Sca-1+ Lineage- hematopoietic stem cells (KSL cells) into precursor B cells, whereas BMMSCs from WT mice did. The impaired differentiation of KSL cells correlated with the reduced production of CXCL12/stromal cell-derived factor-1 by the cocultured C/EBPβ-deficient BMMSCs. The ability of C/EBPβ-deficient BMMSCs to undergo osteogenic and adipogenic differentiation was also defective. The survival of leukemic precursor B cells was poorer when they were cocultured with C/EBPβ- deficient BMMSCs than when they were cocultured with WT BMMSCs. These results indicate that C/EBPβ expressed by BMMSCs plays a crucial role in early B-cell lymphopoiesis.

Original languageEnglish
Pages (from-to)730-740
Number of pages11
JournalSTEM CELLS
Volume32
Issue number3
DOIs
Publication statusPublished - Mar 1 2014
Externally publishedYes

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Lymphopoiesis
CCAAT-Enhancer-Binding Proteins
Mesenchymal Stromal Cells
B-Lymphocytes
B-Lymphoid Precursor Cells
Knockout Mice
Hematopoietic Stem Cells
Bone Marrow Cells
Bone Marrow
Chemokine CXCL12

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

CCAAT/enhancer-binding protein β expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis. / Yoshioka, Satoshi; Miura, Yasuo; Yao, Hisayuki; Satake, Sakiko; Hayashi, Yoshihiro; Tamura, Akihiro; Hishita, Terutoshi; Ichinohe, Tatsuo; Hirai, Hideyo; Takaor-Kondo, Akifumi; Maekawa, Taira.

In: STEM CELLS, Vol. 32, No. 3, 01.03.2014, p. 730-740.

Research output: Contribution to journalArticle

Yoshioka, S, Miura, Y, Yao, H, Satake, S, Hayashi, Y, Tamura, A, Hishita, T, Ichinohe, T, Hirai, H, Takaor-Kondo, A & Maekawa, T 2014, 'CCAAT/enhancer-binding protein β expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis', STEM CELLS, vol. 32, no. 3, pp. 730-740. https://doi.org/10.1002/stem.1555
Yoshioka, Satoshi ; Miura, Yasuo ; Yao, Hisayuki ; Satake, Sakiko ; Hayashi, Yoshihiro ; Tamura, Akihiro ; Hishita, Terutoshi ; Ichinohe, Tatsuo ; Hirai, Hideyo ; Takaor-Kondo, Akifumi ; Maekawa, Taira. / CCAAT/enhancer-binding protein β expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis. In: STEM CELLS. 2014 ; Vol. 32, No. 3. pp. 730-740.
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