CD25+CD4+ regulatory T cells exert in vitro suppressive activity independent of CTLA-4

Hiroshi Kataoka, Shigekazu Takahashi, Kan Takase, Sho Yamasaki, Tadashi Yokosuka, Takao Koike, Takashi Saito

    Research output: Contribution to journalArticlepeer-review

    68 Citations (Scopus)

    Abstract

    Cytotoxic T lymphocyte antigen-4 (CTLA-4) is constitutively expressed on CD25+CD4+ regulatory T cells (Treg) and is suggested to play a role in Treg-mediated suppression. However, the results of analysis with anti-CTLA-4 have been controversial. We addressed this issue by analyzing mice over-expressing or deficient in CTLA-4. For over-expression, CTLA-4 transgenic mice expressing a full-length (FL) or a truncated (TL) mutant of CTLA-4 were analyzed. FL T cells expressed similar levels of CTLA-4 to Treg, whereas TL T cells expressed much higher levels on the cell surface. The number of Treg in both mice was decreased, although Foxp3 expression was not altered. Treg from both mice exerted suppressive activity, whereas CD25- T cells from FL mice showed no suppression. Furthermore, CD25+CD4 thymocytes from young CTLA-4-deficient mice were analyzed and found to exhibit suppressive activity. These results indicate that Treg exert in vitro suppressive activity independent of CTLA-4 expression.

    Original languageEnglish
    Pages (from-to)421-427
    Number of pages7
    JournalInternational immunology
    Volume17
    Issue number4
    DOIs
    Publication statusPublished - Apr 2005

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

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