Abstract
Objective To address the role of cord blood (CB) CD25+CD4 + T cells, the gene expressions and function of this subset were analyzed. Materials and methods CD25+CD4+ T cells fractionated from CB of term and preterm infants were subjected to flow cytometry, quantitative polymerase chain reaction analysis for cytokines, costimulatory molecules, and transcription factors, and functional assays. Results Human preterm CB contained a high proportion of CD25+CD4 + T cells that declined with gestational age to the level of adult peripheral blood (PB). CD25+ or CD25-CD4+ T cells in CB had a higher frequency of CD45RA+ and CD38+ cells than in PB. CB CD25+CD4+ T cells less frequently expressed CD45RO, CD71, and HLA-DR than PB CD25+CD4+ T cells, despite similar expressions on CB and PB CD25-CD4+ T cells. No expression of IL-10, transforming growth factor-β, interleukin-4, and interferon-γ mRNA differed between CB CD25 +CD4+ and CD25-CD4+ T cells, in contrast to the high interleukin-10 expression in PB CD25+CD4 + T cells. CTLA-4 was more transcribed in CB and PB CD25 +CD4+ T cells than in the counterpart CD25 -CD4+ T cells. CD28 or ICOS was similarly expressed in CB and PB T cells. CB CD25+CD4+ T cells effectively suppressed the proliferation of CB CD25-CD4+ T cells in a dose-dependent manner. Human CB and PB CD25+CD4+ T cells preferentially transcribed Foxp3, which governs the regulatory function of this subset in mice. Conclusions These results suggest that CB contains CD25 +CD4+ regulatory T cells as a functionally mature population with naive phenotype. This subset may naturally arise and decline in fetus to play a potential immunoregulatory role in intrauterine life.
| Original language | English |
|---|---|
| Pages (from-to) | 622-629 |
| Number of pages | 8 |
| Journal | Experimental Hematology |
| Volume | 32 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2004 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Hematology
- Genetics
- Cell Biology
- Cancer Research
Cite this
CD25+CD4+ T cells in human cord blood : An immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene. / Takahata, Yasushi; Nomura, Akihiko; Takada, Hidetoshi; Ohga, Shouichi; Furuno, Kenji; Hikino, Shunji; Nakayama, Hideki; Sakaguchi, Shimon; Hara, Toshiro.
In: Experimental Hematology, Vol. 32, No. 7, 01.07.2004, p. 622-629.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD25+CD4+ T cells in human cord blood
T2 - An immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene
AU - Takahata, Yasushi
AU - Nomura, Akihiko
AU - Takada, Hidetoshi
AU - Ohga, Shouichi
AU - Furuno, Kenji
AU - Hikino, Shunji
AU - Nakayama, Hideki
AU - Sakaguchi, Shimon
AU - Hara, Toshiro
PY - 2004/7/1
Y1 - 2004/7/1
N2 - Objective To address the role of cord blood (CB) CD25+CD4 + T cells, the gene expressions and function of this subset were analyzed. Materials and methods CD25+CD4+ T cells fractionated from CB of term and preterm infants were subjected to flow cytometry, quantitative polymerase chain reaction analysis for cytokines, costimulatory molecules, and transcription factors, and functional assays. Results Human preterm CB contained a high proportion of CD25+CD4 + T cells that declined with gestational age to the level of adult peripheral blood (PB). CD25+ or CD25-CD4+ T cells in CB had a higher frequency of CD45RA+ and CD38+ cells than in PB. CB CD25+CD4+ T cells less frequently expressed CD45RO, CD71, and HLA-DR than PB CD25+CD4+ T cells, despite similar expressions on CB and PB CD25-CD4+ T cells. No expression of IL-10, transforming growth factor-β, interleukin-4, and interferon-γ mRNA differed between CB CD25 +CD4+ and CD25-CD4+ T cells, in contrast to the high interleukin-10 expression in PB CD25+CD4 + T cells. CTLA-4 was more transcribed in CB and PB CD25 +CD4+ T cells than in the counterpart CD25 -CD4+ T cells. CD28 or ICOS was similarly expressed in CB and PB T cells. CB CD25+CD4+ T cells effectively suppressed the proliferation of CB CD25-CD4+ T cells in a dose-dependent manner. Human CB and PB CD25+CD4+ T cells preferentially transcribed Foxp3, which governs the regulatory function of this subset in mice. Conclusions These results suggest that CB contains CD25 +CD4+ regulatory T cells as a functionally mature population with naive phenotype. This subset may naturally arise and decline in fetus to play a potential immunoregulatory role in intrauterine life.
AB - Objective To address the role of cord blood (CB) CD25+CD4 + T cells, the gene expressions and function of this subset were analyzed. Materials and methods CD25+CD4+ T cells fractionated from CB of term and preterm infants were subjected to flow cytometry, quantitative polymerase chain reaction analysis for cytokines, costimulatory molecules, and transcription factors, and functional assays. Results Human preterm CB contained a high proportion of CD25+CD4 + T cells that declined with gestational age to the level of adult peripheral blood (PB). CD25+ or CD25-CD4+ T cells in CB had a higher frequency of CD45RA+ and CD38+ cells than in PB. CB CD25+CD4+ T cells less frequently expressed CD45RO, CD71, and HLA-DR than PB CD25+CD4+ T cells, despite similar expressions on CB and PB CD25-CD4+ T cells. No expression of IL-10, transforming growth factor-β, interleukin-4, and interferon-γ mRNA differed between CB CD25 +CD4+ and CD25-CD4+ T cells, in contrast to the high interleukin-10 expression in PB CD25+CD4 + T cells. CTLA-4 was more transcribed in CB and PB CD25 +CD4+ T cells than in the counterpart CD25 -CD4+ T cells. CD28 or ICOS was similarly expressed in CB and PB T cells. CB CD25+CD4+ T cells effectively suppressed the proliferation of CB CD25-CD4+ T cells in a dose-dependent manner. Human CB and PB CD25+CD4+ T cells preferentially transcribed Foxp3, which governs the regulatory function of this subset in mice. Conclusions These results suggest that CB contains CD25 +CD4+ regulatory T cells as a functionally mature population with naive phenotype. This subset may naturally arise and decline in fetus to play a potential immunoregulatory role in intrauterine life.
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UR - http://www.scopus.com/inward/citedby.url?scp=3042792942&partnerID=8YFLogxK
U2 - 10.1016/j.exphem.2004.03.012
DO - 10.1016/j.exphem.2004.03.012
M3 - Article
C2 - 15246158
AN - SCOPUS:3042792942
VL - 32
SP - 622
EP - 629
JO - Experimental Hematology
JF - Experimental Hematology
SN - 0301-472X
IS - 7
ER -