CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages

Sahoko Matsuoka, Yasuhiro Ebihara, Ming Jiang Xu, Takefumi Ishii, Daisuke Sugiyama, Hiroshi Yoshino, Takahiro Ueda, Atsushi Manabe, Ryuhei Tanaka, Yasuo Ikeda, Tatsutoshi Nakahata, Kohichiro Tsuji

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The CD34 antigen serves as an important marker for primitive hematopoietic cells in therapeutic transplantation of hematopoietic stem cells (HSC) and gene therapy, but it has remained an open question as to whether or not most HSC express CD34. Using a competitive long-term reconstitution assay, the results of this study confirm developmental changes in CD34 expression on murine HSC. In fetuses and neonates, CD34 was expressed on Lin-c-Kit+ long-term repopulating HSC of bone marrow (BM), liver, and spleen. However, CD34 expression on HSC decreased with aging, and in mice older than 10 weeks, HSC were most enriched in the Lin-c-Kit+CD34- marrow cell fraction. A second transplantation was performed from primary recipients who were transplanted with neonatal Lin-c-Kit+ CD34high HSC marrow. Although donor-type HSC resided in CD34-Expressing cell fraction in BM cells of the first recipients 4 weeks after the first transplantation, the stem cell activity had shifted to Lin-c-Kit+CD34- cells after 16 weeks, indicating that adult Lin-c-Kit+CD34- HSC are the progeny of neonatal CD34-expresssing HSC. Assays for colony-forming cells showed that hematopoietic progenitor cells, unlike HSC, continue to express CD34 throughout murine development. The present findings are important because the clinical application of HSC can be extended, in particular as related to CD34-Enriched HSC and umbilical cord blood HSC.

Original languageEnglish
Pages (from-to)419-425
Number of pages7
JournalBlood
Volume97
Issue number2
DOIs
Publication statusPublished - Jan 15 2001

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Hematopoietic Stem Cells
Stem cells
Bone Marrow
Assays
Bone
Transplantation
CD34 Antigens
Gene therapy
Stem Cell Transplantation
Cell- and Tissue-Based Therapy
Fetal Blood
Bone Marrow Cells
Genetic Therapy
Liver
Fetus
Spleen
Blood

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages. / Matsuoka, Sahoko; Ebihara, Yasuhiro; Xu, Ming Jiang; Ishii, Takefumi; Sugiyama, Daisuke; Yoshino, Hiroshi; Ueda, Takahiro; Manabe, Atsushi; Tanaka, Ryuhei; Ikeda, Yasuo; Nakahata, Tatsutoshi; Tsuji, Kohichiro.

In: Blood, Vol. 97, No. 2, 15.01.2001, p. 419-425.

Research output: Contribution to journalArticle

Matsuoka, S, Ebihara, Y, Xu, MJ, Ishii, T, Sugiyama, D, Yoshino, H, Ueda, T, Manabe, A, Tanaka, R, Ikeda, Y, Nakahata, T & Tsuji, K 2001, 'CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages', Blood, vol. 97, no. 2, pp. 419-425. https://doi.org/10.1182/blood.V97.2.419
Matsuoka, Sahoko ; Ebihara, Yasuhiro ; Xu, Ming Jiang ; Ishii, Takefumi ; Sugiyama, Daisuke ; Yoshino, Hiroshi ; Ueda, Takahiro ; Manabe, Atsushi ; Tanaka, Ryuhei ; Ikeda, Yasuo ; Nakahata, Tatsutoshi ; Tsuji, Kohichiro. / CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages. In: Blood. 2001 ; Vol. 97, No. 2. pp. 419-425.
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