Abstract
We studied local events in the popliteal lymph nodes of CD4-deficient mice following foot pad injection with an MMTV strain which carries the gene for a Vβ14-specific superantigen. Injection of the Vβ14-specific MMTV induced vigorous expansion of Vβ14+ CD4+ T cells and B cells in their lymph nodes of CD4+/- heterozygous control mice. On the other hand, CD4-/- mice injected with the MMTV showed a proliferation of Vβ14+ T cells among the population of TCRαβ+ CD4-CD8- T cells, although to a lesser extent. This phenomenon was not accompanied by vigorous B cell expansion. A PCR assay revelated that the MMTV definitely infected the lymph nodes cells of the CD4-/- mouse. However, the infectivity of the MMTV in CD4-/- mice was approximately 20 times lower than that in CD4+/- mice. These findings indicate that, in MMTV infection of CD4-deficient mice, the superantigen-reactive T cells among the population of TCRαβ+ CD4(z)CD8(x) T cells substitute for the superantigen-reactive CD4+ T cells of normal mice, and that the absence of CD4 molecules decreased the infectivity of MMTV because of insufficient expansion of the superantigen-reactive T cells.
| Original language | English |
|---|---|
| Pages (from-to) | 376-384 |
| Number of pages | 9 |
| Journal | Immunobiology |
| Volume | 195 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 1996 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
- Hematology
Cite this
CD4 expression is important but not essential for infection with exogenous mouse mammary tumor virus. / Ando, Yoshohiro; Wajjwalku, Worawidh; Kishihara, Kenji; Arai, Toshiyuki; Niimi, Naoya; Hiromatsu, Kenji; Morishima, Tsuneo; Yoshikai, Yasunobu.
In: Immunobiology, Vol. 195, No. 3, 08.1996, p. 376-384.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD4 expression is important but not essential for infection with exogenous mouse mammary tumor virus
AU - Ando, Yoshohiro
AU - Wajjwalku, Worawidh
AU - Kishihara, Kenji
AU - Arai, Toshiyuki
AU - Niimi, Naoya
AU - Hiromatsu, Kenji
AU - Morishima, Tsuneo
AU - Yoshikai, Yasunobu
PY - 1996/8
Y1 - 1996/8
N2 - We studied local events in the popliteal lymph nodes of CD4-deficient mice following foot pad injection with an MMTV strain which carries the gene for a Vβ14-specific superantigen. Injection of the Vβ14-specific MMTV induced vigorous expansion of Vβ14+ CD4+ T cells and B cells in their lymph nodes of CD4+/- heterozygous control mice. On the other hand, CD4-/- mice injected with the MMTV showed a proliferation of Vβ14+ T cells among the population of TCRαβ+ CD4-CD8- T cells, although to a lesser extent. This phenomenon was not accompanied by vigorous B cell expansion. A PCR assay revelated that the MMTV definitely infected the lymph nodes cells of the CD4-/- mouse. However, the infectivity of the MMTV in CD4-/- mice was approximately 20 times lower than that in CD4+/- mice. These findings indicate that, in MMTV infection of CD4-deficient mice, the superantigen-reactive T cells among the population of TCRαβ+ CD4(z)CD8(x) T cells substitute for the superantigen-reactive CD4+ T cells of normal mice, and that the absence of CD4 molecules decreased the infectivity of MMTV because of insufficient expansion of the superantigen-reactive T cells.
AB - We studied local events in the popliteal lymph nodes of CD4-deficient mice following foot pad injection with an MMTV strain which carries the gene for a Vβ14-specific superantigen. Injection of the Vβ14-specific MMTV induced vigorous expansion of Vβ14+ CD4+ T cells and B cells in their lymph nodes of CD4+/- heterozygous control mice. On the other hand, CD4-/- mice injected with the MMTV showed a proliferation of Vβ14+ T cells among the population of TCRαβ+ CD4-CD8- T cells, although to a lesser extent. This phenomenon was not accompanied by vigorous B cell expansion. A PCR assay revelated that the MMTV definitely infected the lymph nodes cells of the CD4-/- mouse. However, the infectivity of the MMTV in CD4-/- mice was approximately 20 times lower than that in CD4+/- mice. These findings indicate that, in MMTV infection of CD4-deficient mice, the superantigen-reactive T cells among the population of TCRαβ+ CD4(z)CD8(x) T cells substitute for the superantigen-reactive CD4+ T cells of normal mice, and that the absence of CD4 molecules decreased the infectivity of MMTV because of insufficient expansion of the superantigen-reactive T cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029792712&partnerID=8YFLogxK
U2 - 10.1016/S0171-2985(96)80053-2
DO - 10.1016/S0171-2985(96)80053-2
M3 - Article
C2 - 8877410
AN - SCOPUS:0029792712
VL - 195
SP - 376
EP - 384
JO - Immunobiology
JF - Immunobiology
SN - 0171-2985
IS - 3
ER -