CD4 T cell-intrinsic IL-2 signaling differentially affects Th1 and Th17 development

Kenjiro Fujimura, Akiko Oyamada, Yukihide Iwamoto, Yasunobu Yoshikai, Hisakata Yamada

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

IL-2 signaling is involved in clonal expansion of antigen-specific CD4 T cells. IL-2 is also reported to promote Th1 but inhibit Th17 differentiation, although in vivo relevance remains unclear. In addition, IL-2-dependent Foxp3+ CD4 Tregs suppress T cell proliferation, complicating the in vivo role of IL-2 in the development of Th cell responses. To elucidate the roles of cell-intrinsic IL-2 signaling in CD4 T cells, we cotransferred TCR-Tg CD4 T cells from IL-2Ra (CD25)-deficient and WT mice and analyzed development of antigen-specific Th1 and Th17 responses. It was revealed that Th17 development of antigen-specific CD4 T cells was largely unaffected, whereas Th1 development was impaired by the lack of IL-2 signaling. Similar data were obtained from mixed BM chimera experiments using BM cells from CD25-deficient and WT mice. In addition, although in vitro blockade of IL-2 during Th17 development greatly increased the percentages of Th17 cells, it did not affect their numbers, indicating that in vitro Th17 development is also IL-2-inde-pendent. Th1 development was dependent on IL-2 in vitro as well. Thus, our data suggest that cell-intrinsic IL-2 signaling is critical for Th1 development but plays a limited role in Th17 development in vitro as well as in vivo.

Original languageEnglish
Pages (from-to)271-279
Number of pages9
JournalJournal of Leukocyte Biology
Volume94
Issue number2
DOIs
Publication statusPublished - Aug 1 2013

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Interleukin-2
T-Lymphocytes
CD4 Antigens
Th17 Cells
Cell Proliferation
Antigens
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

CD4 T cell-intrinsic IL-2 signaling differentially affects Th1 and Th17 development. / Fujimura, Kenjiro; Oyamada, Akiko; Iwamoto, Yukihide; Yoshikai, Yasunobu; Yamada, Hisakata.

In: Journal of Leukocyte Biology, Vol. 94, No. 2, 01.08.2013, p. 271-279.

Research output: Contribution to journalArticle

Fujimura, Kenjiro ; Oyamada, Akiko ; Iwamoto, Yukihide ; Yoshikai, Yasunobu ; Yamada, Hisakata. / CD4 T cell-intrinsic IL-2 signaling differentially affects Th1 and Th17 development. In: Journal of Leukocyte Biology. 2013 ; Vol. 94, No. 2. pp. 271-279.
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