CD95 (Fas) ligand expression of Epstein-Barr virus (EBV)-infected lymphocytes

A possible mechanism of immune evasion in chronic active EBV infection

Koichi Ohshima, Junji Suzumiya, Midori Sugihara, Seiho Nagafuchi, Shoichi Ohga, Masahiro Kikuchi

Research output: Contribution to journalArticle

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Abstract

The Epstein-Barr virus (EBV) induces infectious mononucleosis (IM) and can be associated with chronic active EBV infection (CAEBV). Cytotoxic T lymphocytes (CTL) play an important role in excluding EBV-infected cells. Two cytotoxic mechanisms of CTL have been demonstrated: one perforin/granzyme- based and the other Fas (CD95)/Fas ligand (FasL)-based. To clarify these two pathways in CAEBV, we analyzed six patients with CAEBV and four patients with IM using immunohistochemical staining of the lymph nodes. In both CAEBV and IM, CD8+ T-cells increased in number, but CD56+ natural killer cells were rare. In four of six cases with CAEBV, approximately half the lymphocytes were positive for T cell-restricted intracellular antigens (TIA-1), which were recognized by the cytolytic granules of CTL. In IM, the number of TIA-1 positive cells was smaller than that in CAEBV. Fas-positive lymphocytes were frequently encountered in both CAEBV and IM. However, FasL-positive lymphocytes increased in three of six patients with CAEBV, but not in patients with IM. Except for one case with CAEBV, the number of perforin- and/or granzyme-positive cells was small in number in both CAEBV and IM cases. In double-staining FasL and EBV in situ hybridization, FasL-positive EBV-infected lymphocytes were detected in CAEBV but not in IM. In CAEBV, the Fas/FasL pathway and not perforin pathways appears to play an important role in the pathogenesis. The data suggest that EBV-infected lymphocytes may evade immune attack through the expression of FasL.

Original languageEnglish
Pages (from-to)9-13
Number of pages5
JournalPathology International
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

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Immune Evasion
Epstein-Barr Virus Infections
Fas Ligand Protein
Human Herpesvirus 4
Infectious Mononucleosis
Lymphocytes
Perforin
Cytotoxic T-Lymphocytes
Granzymes
Staining and Labeling
T-Lymphocytes
Natural Killer Cells
In Situ Hybridization

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

CD95 (Fas) ligand expression of Epstein-Barr virus (EBV)-infected lymphocytes : A possible mechanism of immune evasion in chronic active EBV infection. / Ohshima, Koichi; Suzumiya, Junji; Sugihara, Midori; Nagafuchi, Seiho; Ohga, Shoichi; Kikuchi, Masahiro.

In: Pathology International, Vol. 49, No. 1, 01.01.1999, p. 9-13.

Research output: Contribution to journalArticle

Ohshima, Koichi ; Suzumiya, Junji ; Sugihara, Midori ; Nagafuchi, Seiho ; Ohga, Shoichi ; Kikuchi, Masahiro. / CD95 (Fas) ligand expression of Epstein-Barr virus (EBV)-infected lymphocytes : A possible mechanism of immune evasion in chronic active EBV infection. In: Pathology International. 1999 ; Vol. 49, No. 1. pp. 9-13.
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