CD95 (Fas) may control the expansion of activated T cells after elimination of bacteria in murine listeriosis

Y. Fuse, H. Nishimura, K. Maeda, Y. Yoshikai

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    CD95 (Fas) is known to mediate activation-induced T-cell death by apoptosis. To understand the role of CD95 during the course of bacterial infection, we examined the kinetics of αβ and γδ T cells in the peritoneal cavities and livers of 5-week-old CD95-defective MRL/lpr mice after an intraperitoneal infection with Listeria monocytogenes. The number of bacteria in the spleen decreased to an undetectable level by day 10 after infection with 7 x l03 Listeria cells similar to the number in MRL/+/+ mice. The number of αβ T cells expressing CD44 and CD95 reached a maximum in the peritoneal cavity on day 6 after listerial infection and thereafter decreased gradually in MRL/+/+ mice, whereas CD44+ αβ T cells without CD95 expression continued to increase throughout the course of listerial infection in MRL/lpr mice. Freshly isolated T cells from MRL/+/+ mice infected with L. monocytogenes 10 days previously showed DNA fragmentation with apoptosis, whereas such fragmentation was not prominent in T cells from infected MRL/lpr mice. In correlation with the increased number of CD44+ αβ T cells, Listeria-specific T-cell proliferation of peritoneal exudate cells was significantly greater in MRL/lpr mice than in MRL/+/+ mice on day l0 after listerial infection. In contrast to αβ T cells, γδ T cells increased in number only transiently in the peritoneal cavity and liver after listerial infection in both MRL/lpr mice and MRL/+/+ mice. These results suggest that CD95-mediated cell death with apoptosis may be involved in termination of the αβ-T-cell-mediated immune response after the battle against L. monocytogenes has been won, whereas γδ T cells may undergo apoptosis independently of CD95 during the course of listerial infection.

    Original languageEnglish
    Pages (from-to)1883-1891
    Number of pages9
    JournalInfection and Immunity
    Volume65
    Issue number5
    Publication statusPublished - May 17 1997

    Fingerprint

    Listeriosis
    Bacteria
    T-Lymphocytes
    Inbred MRL lpr Mouse
    Infection
    Peritoneal Cavity
    Listeria monocytogenes
    Apoptosis
    Listeria
    Cell Death
    Liver
    DNA Fragmentation
    Exudates and Transudates
    Bacterial Infections
    Spleen

    All Science Journal Classification (ASJC) codes

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

    Cite this

    CD95 (Fas) may control the expansion of activated T cells after elimination of bacteria in murine listeriosis. / Fuse, Y.; Nishimura, H.; Maeda, K.; Yoshikai, Y.

    In: Infection and Immunity, Vol. 65, No. 5, 17.05.1997, p. 1883-1891.

    Research output: Contribution to journalArticle

    Fuse, Y, Nishimura, H, Maeda, K & Yoshikai, Y 1997, 'CD95 (Fas) may control the expansion of activated T cells after elimination of bacteria in murine listeriosis', Infection and Immunity, vol. 65, no. 5, pp. 1883-1891.
    Fuse, Y. ; Nishimura, H. ; Maeda, K. ; Yoshikai, Y. / CD95 (Fas) may control the expansion of activated T cells after elimination of bacteria in murine listeriosis. In: Infection and Immunity. 1997 ; Vol. 65, No. 5. pp. 1883-1891.
    @article{4b9b57cf717b42eaadb7358e2084e021,
    title = "CD95 (Fas) may control the expansion of activated T cells after elimination of bacteria in murine listeriosis",
    abstract = "CD95 (Fas) is known to mediate activation-induced T-cell death by apoptosis. To understand the role of CD95 during the course of bacterial infection, we examined the kinetics of αβ and γδ T cells in the peritoneal cavities and livers of 5-week-old CD95-defective MRL/lpr mice after an intraperitoneal infection with Listeria monocytogenes. The number of bacteria in the spleen decreased to an undetectable level by day 10 after infection with 7 x l03 Listeria cells similar to the number in MRL/+/+ mice. The number of αβ T cells expressing CD44 and CD95 reached a maximum in the peritoneal cavity on day 6 after listerial infection and thereafter decreased gradually in MRL/+/+ mice, whereas CD44+ αβ T cells without CD95 expression continued to increase throughout the course of listerial infection in MRL/lpr mice. Freshly isolated T cells from MRL/+/+ mice infected with L. monocytogenes 10 days previously showed DNA fragmentation with apoptosis, whereas such fragmentation was not prominent in T cells from infected MRL/lpr mice. In correlation with the increased number of CD44+ αβ T cells, Listeria-specific T-cell proliferation of peritoneal exudate cells was significantly greater in MRL/lpr mice than in MRL/+/+ mice on day l0 after listerial infection. In contrast to αβ T cells, γδ T cells increased in number only transiently in the peritoneal cavity and liver after listerial infection in both MRL/lpr mice and MRL/+/+ mice. These results suggest that CD95-mediated cell death with apoptosis may be involved in termination of the αβ-T-cell-mediated immune response after the battle against L. monocytogenes has been won, whereas γδ T cells may undergo apoptosis independently of CD95 during the course of listerial infection.",
    author = "Y. Fuse and H. Nishimura and K. Maeda and Y. Yoshikai",
    year = "1997",
    month = "5",
    day = "17",
    language = "English",
    volume = "65",
    pages = "1883--1891",
    journal = "Infection and Immunity",
    issn = "0019-9567",
    publisher = "American Society for Microbiology",
    number = "5",

    }

    TY - JOUR

    T1 - CD95 (Fas) may control the expansion of activated T cells after elimination of bacteria in murine listeriosis

    AU - Fuse, Y.

    AU - Nishimura, H.

    AU - Maeda, K.

    AU - Yoshikai, Y.

    PY - 1997/5/17

    Y1 - 1997/5/17

    N2 - CD95 (Fas) is known to mediate activation-induced T-cell death by apoptosis. To understand the role of CD95 during the course of bacterial infection, we examined the kinetics of αβ and γδ T cells in the peritoneal cavities and livers of 5-week-old CD95-defective MRL/lpr mice after an intraperitoneal infection with Listeria monocytogenes. The number of bacteria in the spleen decreased to an undetectable level by day 10 after infection with 7 x l03 Listeria cells similar to the number in MRL/+/+ mice. The number of αβ T cells expressing CD44 and CD95 reached a maximum in the peritoneal cavity on day 6 after listerial infection and thereafter decreased gradually in MRL/+/+ mice, whereas CD44+ αβ T cells without CD95 expression continued to increase throughout the course of listerial infection in MRL/lpr mice. Freshly isolated T cells from MRL/+/+ mice infected with L. monocytogenes 10 days previously showed DNA fragmentation with apoptosis, whereas such fragmentation was not prominent in T cells from infected MRL/lpr mice. In correlation with the increased number of CD44+ αβ T cells, Listeria-specific T-cell proliferation of peritoneal exudate cells was significantly greater in MRL/lpr mice than in MRL/+/+ mice on day l0 after listerial infection. In contrast to αβ T cells, γδ T cells increased in number only transiently in the peritoneal cavity and liver after listerial infection in both MRL/lpr mice and MRL/+/+ mice. These results suggest that CD95-mediated cell death with apoptosis may be involved in termination of the αβ-T-cell-mediated immune response after the battle against L. monocytogenes has been won, whereas γδ T cells may undergo apoptosis independently of CD95 during the course of listerial infection.

    AB - CD95 (Fas) is known to mediate activation-induced T-cell death by apoptosis. To understand the role of CD95 during the course of bacterial infection, we examined the kinetics of αβ and γδ T cells in the peritoneal cavities and livers of 5-week-old CD95-defective MRL/lpr mice after an intraperitoneal infection with Listeria monocytogenes. The number of bacteria in the spleen decreased to an undetectable level by day 10 after infection with 7 x l03 Listeria cells similar to the number in MRL/+/+ mice. The number of αβ T cells expressing CD44 and CD95 reached a maximum in the peritoneal cavity on day 6 after listerial infection and thereafter decreased gradually in MRL/+/+ mice, whereas CD44+ αβ T cells without CD95 expression continued to increase throughout the course of listerial infection in MRL/lpr mice. Freshly isolated T cells from MRL/+/+ mice infected with L. monocytogenes 10 days previously showed DNA fragmentation with apoptosis, whereas such fragmentation was not prominent in T cells from infected MRL/lpr mice. In correlation with the increased number of CD44+ αβ T cells, Listeria-specific T-cell proliferation of peritoneal exudate cells was significantly greater in MRL/lpr mice than in MRL/+/+ mice on day l0 after listerial infection. In contrast to αβ T cells, γδ T cells increased in number only transiently in the peritoneal cavity and liver after listerial infection in both MRL/lpr mice and MRL/+/+ mice. These results suggest that CD95-mediated cell death with apoptosis may be involved in termination of the αβ-T-cell-mediated immune response after the battle against L. monocytogenes has been won, whereas γδ T cells may undergo apoptosis independently of CD95 during the course of listerial infection.

    UR - http://www.scopus.com/inward/record.url?scp=0030945162&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0030945162&partnerID=8YFLogxK

    M3 - Article

    C2 - 9125576

    AN - SCOPUS:0030945162

    VL - 65

    SP - 1883

    EP - 1891

    JO - Infection and Immunity

    JF - Infection and Immunity

    SN - 0019-9567

    IS - 5

    ER -