Cdc7-Drf1 is a developmentally regulated protein kinase required for the initiation of vertebrate DNA replication

Tatsuro S. Takahashi, Johannes C. Walter

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

Cdc7, a protein kinase required for the initiation of eukaryotic DNA replication, is activated by a regulatory subunit, Dbf4. A second activator of Cdc7 called Drf1 exists in vertebrates, but its function is unknown. Here, we report that in Xenopus egg extracts, Cdc7-Drf1 is far more abundant than Cdc7-Dbf4, and removal of Drf1 but not Dbf4 severely inhibits phosphorylation of Mcm4 and DNA replication. After gastrulation, when the cell cycle acquires somatic characteristics, Drf1 levels decline sharply and Cdc7-Dbf4 becomes the more abundant kinase. These results identify Drf1 as a developmentally regulated, essential activator of Cdc7 in Xenopus.

Original languageEnglish
Pages (from-to)2295-2300
Number of pages6
JournalGenes and Development
Volume19
Issue number19
DOIs
Publication statusPublished - Oct 1 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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