Abstract
Cdc7, a protein kinase required for the initiation of eukaryotic DNA replication, is activated by a regulatory subunit, Dbf4. A second activator of Cdc7 called Drf1 exists in vertebrates, but its function is unknown. Here, we report that in Xenopus egg extracts, Cdc7-Drf1 is far more abundant than Cdc7-Dbf4, and removal of Drf1 but not Dbf4 severely inhibits phosphorylation of Mcm4 and DNA replication. After gastrulation, when the cell cycle acquires somatic characteristics, Drf1 levels decline sharply and Cdc7-Dbf4 becomes the more abundant kinase. These results identify Drf1 as a developmentally regulated, essential activator of Cdc7 in Xenopus.
| Original language | English |
|---|---|
| Pages (from-to) | 2295-2300 |
| Number of pages | 6 |
| Journal | Genes and Development |
| Volume | 19 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - Oct 1 2005 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Genetics
- Developmental Biology
Cite this
Cdc7-Drf1 is a developmentally regulated protein kinase required for the initiation of vertebrate DNA replication. / Takahashi, Tatsuro S.; Walter, Johannes C.
In: Genes and Development, Vol. 19, No. 19, 01.10.2005, p. 2295-2300.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cdc7-Drf1 is a developmentally regulated protein kinase required for the initiation of vertebrate DNA replication
AU - Takahashi, Tatsuro S.
AU - Walter, Johannes C.
PY - 2005/10/1
Y1 - 2005/10/1
N2 - Cdc7, a protein kinase required for the initiation of eukaryotic DNA replication, is activated by a regulatory subunit, Dbf4. A second activator of Cdc7 called Drf1 exists in vertebrates, but its function is unknown. Here, we report that in Xenopus egg extracts, Cdc7-Drf1 is far more abundant than Cdc7-Dbf4, and removal of Drf1 but not Dbf4 severely inhibits phosphorylation of Mcm4 and DNA replication. After gastrulation, when the cell cycle acquires somatic characteristics, Drf1 levels decline sharply and Cdc7-Dbf4 becomes the more abundant kinase. These results identify Drf1 as a developmentally regulated, essential activator of Cdc7 in Xenopus.
AB - Cdc7, a protein kinase required for the initiation of eukaryotic DNA replication, is activated by a regulatory subunit, Dbf4. A second activator of Cdc7 called Drf1 exists in vertebrates, but its function is unknown. Here, we report that in Xenopus egg extracts, Cdc7-Drf1 is far more abundant than Cdc7-Dbf4, and removal of Drf1 but not Dbf4 severely inhibits phosphorylation of Mcm4 and DNA replication. After gastrulation, when the cell cycle acquires somatic characteristics, Drf1 levels decline sharply and Cdc7-Dbf4 becomes the more abundant kinase. These results identify Drf1 as a developmentally regulated, essential activator of Cdc7 in Xenopus.
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UR - http://www.scopus.com/inward/citedby.url?scp=25844447786&partnerID=8YFLogxK
U2 - 10.1101/gad.1339805
DO - 10.1101/gad.1339805
M3 - Article
C2 - 16204181
AN - SCOPUS:25844447786
VL - 19
SP - 2295
EP - 2300
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 19
ER -