Cdh1 degradation is mediated by APC/C–Cdh1 and SCF–Cdc4 in budding yeast

Masayoshi Nagai, Atsuko Shibata, Takashi Ushimaru

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Cdh1, a substrate-recognition subunit of anaphase-promoting complex/cyclosome (APC/C), is a tumor suppressor, and it is downregulated in various tumor cells in humans. APC/C–Cdh1 is activated from late M phase to G1 phase by antagonizing Cdk1-mediated inhibitory phosphorylation. However, how Cdh1 protein levels are properly regulated is ill-defined. Here we show that Cdh1 is degraded via APC/C–Cdh1 and Skp1–Cullin1–F-box (SCF)–Cdc4 in the budding yeast Saccharomyces cerevisiae. Cdh1 degradation was promoted by forced localization of Cdh1 into the nucleus, where APC/C and SCF are present. Cdk1 promoted APC/C–Cdh1-mediated Cdh1 degradation, whereas polo kinase Cdc5 elicited SCF–Cdc4-mediated degradation. Thus, Cdh1 degradation is controlled via multiple pathways.

Original languageEnglish
Pages (from-to)932-938
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume506
Issue number4
DOIs
Publication statusPublished - Dec 2 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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