CDK phosphorylation regulates Mcm3 degradation in budding yeast

Kaori Yamamoto, Nishiho Makino, Masayoshi Nagai, Hiroyuki Araki, Takashi Ushimaru

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Accurate regulation of activity and level of the MCM complex is critical for precise DNA replication and genome transmission. Cyclin-dependent kinase (CDK) negatively regulates nuclear localization of the MCM complex via phosphorylation of the Mcm3 subunit. More recently, we found that Mcm3 is degraded via the Skp1–Cullin–F-box (SCF)–proteasome axis in budding yeast. However, how Mcm3 degradation is regulated is largely unknown. Here, we show that CDK represses Mcm3 degradation. Phosphorylated Mcm3 was excluded from the nucleus, where SCF is predominantly located, although CDK-mediated phosphorylation itself generated a phosphodegron of Mcm3, stimulating the degradation of Mcm3 resident in the nucleus. Thus, CDK negatively regulated nuclear MCM levels by exclusion from the nucleus and degradation in the nucleus via Mcm3 phosphorylation. We will discuss the physiological importance of Mcm3 degradation.

Original languageEnglish
Pages (from-to)680-684
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume506
Issue number3
DOIs
Publication statusPublished - Nov 30 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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