Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex

Hiromi Maekawa, Elmar Schiebel

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, associates only with the SPB in the mother cell and so prevents Kar9 binding to this SPB. However, here we show that the nonoverexpressed Clb4 associates with the budward-directed SPB through Kar9. Cdc28-Clb4 then uses Kar9 as a carrier to move from this SPB to the plus ends of astral microtubules, where Cdc28-Clb4 regulates the interactions between microtubule ends and subdomains of the bud cortex. In the absence of Cdc28-Clb4 activity (G1/S phase), astral microtubules interact with the bud tip in a manner dependent on actin, Myo2, and Kar9. Coincidentally with reaching the bud cortex in S phase, Cdc28-Clb4 facilitates the dissociation of the microtubule bud tip interaction and their capture by the bud neck. This transition prevents the preanaphase spindle from becoming prematurely pulled into the bud. Thus, Cdc28-Clb4 facilitates spindle alignment by regulating the interaction of astral microtubules with subdomains of the bud cortex.

Original languageEnglish
Pages (from-to)1709-1724
Number of pages16
JournalGenes and Development
Volume18
Issue number14
DOIs
Publication statusPublished - Jul 15 2004
Externally publishedYes

Fingerprint

Spindle Pole Bodies
Microtubules
S Phase
Cyclins
Cyclin-Dependent Kinases
G1 Phase
Actins
Cell Cycle
Carrier Proteins
Stem Cells
Yeasts

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex. / Maekawa, Hiromi; Schiebel, Elmar.

In: Genes and Development, Vol. 18, No. 14, 15.07.2004, p. 1709-1724.

Research output: Contribution to journalArticle

@article{f9b6b609169d4c6699069dc8751e420f,
title = "Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex",
abstract = "As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, associates only with the SPB in the mother cell and so prevents Kar9 binding to this SPB. However, here we show that the nonoverexpressed Clb4 associates with the budward-directed SPB through Kar9. Cdc28-Clb4 then uses Kar9 as a carrier to move from this SPB to the plus ends of astral microtubules, where Cdc28-Clb4 regulates the interactions between microtubule ends and subdomains of the bud cortex. In the absence of Cdc28-Clb4 activity (G1/S phase), astral microtubules interact with the bud tip in a manner dependent on actin, Myo2, and Kar9. Coincidentally with reaching the bud cortex in S phase, Cdc28-Clb4 facilitates the dissociation of the microtubule bud tip interaction and their capture by the bud neck. This transition prevents the preanaphase spindle from becoming prematurely pulled into the bud. Thus, Cdc28-Clb4 facilitates spindle alignment by regulating the interaction of astral microtubules with subdomains of the bud cortex.",
author = "Hiromi Maekawa and Elmar Schiebel",
year = "2004",
month = "7",
day = "15",
doi = "10.1101/gad.298704",
language = "English",
volume = "18",
pages = "1709--1724",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "14",

}

TY - JOUR

T1 - Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex

AU - Maekawa, Hiromi

AU - Schiebel, Elmar

PY - 2004/7/15

Y1 - 2004/7/15

N2 - As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, associates only with the SPB in the mother cell and so prevents Kar9 binding to this SPB. However, here we show that the nonoverexpressed Clb4 associates with the budward-directed SPB through Kar9. Cdc28-Clb4 then uses Kar9 as a carrier to move from this SPB to the plus ends of astral microtubules, where Cdc28-Clb4 regulates the interactions between microtubule ends and subdomains of the bud cortex. In the absence of Cdc28-Clb4 activity (G1/S phase), astral microtubules interact with the bud tip in a manner dependent on actin, Myo2, and Kar9. Coincidentally with reaching the bud cortex in S phase, Cdc28-Clb4 facilitates the dissociation of the microtubule bud tip interaction and their capture by the bud neck. This transition prevents the preanaphase spindle from becoming prematurely pulled into the bud. Thus, Cdc28-Clb4 facilitates spindle alignment by regulating the interaction of astral microtubules with subdomains of the bud cortex.

AB - As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, associates only with the SPB in the mother cell and so prevents Kar9 binding to this SPB. However, here we show that the nonoverexpressed Clb4 associates with the budward-directed SPB through Kar9. Cdc28-Clb4 then uses Kar9 as a carrier to move from this SPB to the plus ends of astral microtubules, where Cdc28-Clb4 regulates the interactions between microtubule ends and subdomains of the bud cortex. In the absence of Cdc28-Clb4 activity (G1/S phase), astral microtubules interact with the bud tip in a manner dependent on actin, Myo2, and Kar9. Coincidentally with reaching the bud cortex in S phase, Cdc28-Clb4 facilitates the dissociation of the microtubule bud tip interaction and their capture by the bud neck. This transition prevents the preanaphase spindle from becoming prematurely pulled into the bud. Thus, Cdc28-Clb4 facilitates spindle alignment by regulating the interaction of astral microtubules with subdomains of the bud cortex.

UR - http://www.scopus.com/inward/record.url?scp=3142673447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142673447&partnerID=8YFLogxK

U2 - 10.1101/gad.298704

DO - 10.1101/gad.298704

M3 - Article

C2 - 15256500

AN - SCOPUS:3142673447

VL - 18

SP - 1709

EP - 1724

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 14

ER -